{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:04:39Z","timestamp":1791209079704,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,6,30]],"date-time":"2004-06-30T00:00:00Z","timestamp":1088553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Phys Anthropol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the course of a molecular genetic investigation of a double inhumation, presumably a mother\/child burial from Aschheim (Upper Bavaria, 6th century A.D.), which included analysis of mitochondrial DNA, molecular sexing, and polymorphic nuclear DNA, <jats:italic>Yersinia pestis<\/jats:italic>\u2010specific DNA was detected. Molecular analyses were performed on DNA extracts obtained from two teeth of one skeleton and four teeth of the other. The use of the primer pair YP12D\/YP11R (Raoult et al. [<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#bib28\">2000<\/jats:ext-link>] Proc. Natl. Acad. Sci. <jats:italic>97:<\/jats:italic>12800\u201312803), able to amplify part of the <jats:italic>Y. pestis<\/jats:italic> plasmid pPCP1 <jats:italic>pla<\/jats:italic> sequence, resulted in amplification products of the expected fragment size. Using BLASTN 2.2.2, the sequences of these amplification products shared 100% identity with that of the modern <jats:italic>Y. pestis pla<\/jats:italic> sequence in GenBank, with the exception of one amplification product which revealed a single base substitution. The application of a \u201csuicide PCR\u201d with the independent primer pair YP11D\/YP10R (Raoult et al. [<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#bib28\">2000<\/jats:ext-link>] Proc. Natl. Acad. Sci. <jats:italic>97:<\/jats:italic>12800\u201312803) resulted in amplification products which shared a 96\u201398% homology with that of the modern <jats:italic>Y. pestis pla<\/jats:italic> sequence in GenBank. The observed deviations were presumably due to miscoding lesions in the template DNA. No modern <jats:italic>Y. pestis<\/jats:italic> DNA was introduced into the institute, and thus no positive controls were carried along. All extraction and PCR controls remained negative. The identification of <jats:italic>Y. pestis<\/jats:italic>\u2010specific DNA sequences in these two skeletons, buried in the second half of the 6th century A.D., constitutes molecularly supported evidence for the presence of <jats:italic>Y. pestis<\/jats:italic>, the causative agent of plague, during the first pandemic recorded. Am J Phys Anthropol, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajpa.10276","type":"journal-article","created":{"date-parts":[[2004,6,30]],"date-time":"2004-06-30T15:07:28Z","timestamp":1088608048000},"page":"48-55","source":"Crossref","is-referenced-by-count":108,"title":["Detection of <i>Yersinia pestis<\/i> DNA in two early medieval skeletal finds from Aschheim (Upper Bavaria, 6th century A.D.)"],"prefix":"10.1002","volume":"126","author":[{"given":"Ingrid","family":"Wiechmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela","family":"Grupe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/290457a0"},{"key":"e_1_2_5_5_1","first-page":"48","volume-title":"Biology of man in history","author":"Biraben JN","year":"1975"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/oa.624"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1128\/jcm.31.3.758-759.1993","article-title":"Rapid and specific identification of Yersinia pestis by using a nested polymerase chain reaction procedure","volume":"31","author":"Campbell J","year":"1993","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-4403(92)90005-N"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.17.4791-a"},{"key":"e_1_2_5_10_1","first-page":"105","article-title":"Immunohistochemistry: a new outlook in histopaleopathology","volume":"71","author":"Fulcheri E","year":"1995","journal-title":"J Biol Res Boll Soc Ital Biol Sper"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2001.580611.x"},{"key":"e_1_2_5_12_1","first-page":"368","article-title":"The retrieval of ancient human DNA sequences","volume":"59","author":"Handt O","year":"1996","journal-title":"Am J Hum Genet"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1128\/jcm.31.6.1511-1514.1993","article-title":"New method for plague surveillance using polymerase chain reaction to detect Yersinia pestis in fleas","volume":"31","author":"Hinnebusch J","year":"1993","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)43176-1"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.23.4793"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.19.5192-5202.1998"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3.1.13"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0379-0738(94)01660-W"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198710153171603"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8644(200001)111:1<5::AID-AJPA2>3.0.CO;2-3"},{"key":"e_1_2_5_21_1","first-page":"43","article-title":"Analysis of ancient mitochondrial DNA from extinct aborigines from Tierra del Fuego\u2010Patagonia","volume":"1","author":"Lalueza Fox C","year":"1996","journal-title":"Ancient Biomol"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004140050161"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/362709a0"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01371335"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390050533"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.10.3445"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.35"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.14.4018"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.220225197"},{"key":"e_1_2_5_30_1","unstructured":"2000 Konrad Theiss Verlag Stuttgart D Reimann K D\u00fcwel A Bartel Vereint in den Tod\u2014Doppelgrab 166\/167 aus Aschheim 83 85"},{"key":"e_1_2_5_31_1","first-page":"185","article-title":"Paleolithic and Neolithic lineages in the European mitochondrial gene pool","volume":"59","author":"Richards M","year":"1996","journal-title":"Am J Hum Genet"},{"key":"e_1_2_5_32_1","volume-title":"Schnelle Silberf\u00e4rbung von Nukleins\u00e4uren. 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