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In one of the first studies of hunter\u2010fisher\u2010gatherer subadults from Eurasia, three Neolithic (8,800\u20105,200 calBP) mortuary sites from southwestern Siberia are analyzed to evaluate hypothesized differences in weaning age between Early versus Late Neolithic groups. An intra\u2010individual sampling methodology is used to compare bone formed at different ages. Collagen samples (<jats:italic>n<\/jats:italic> = 143) from three different growth areas of long bones\u2014the proximal metaphysis, diaphysis, and distal metaphysis\u2014were obtained from 49 subadults aged birth to 10 years. In infants (birth to 3 years, <jats:italic>n<\/jats:italic> = 23) contrasting the \u03b4<jats:sup>15<\/jats:sup>N values of the metaphysis, which contains newer bone, to the \u03b4<jats:sup>15<\/jats:sup>N values of the diaphysis, which contains older bone, permits a more precise determination of breastfeeding\u2010weaning status. In Early and Late Neolithic groups breast milk was the major protein source until the age of 2\u20133 years. However, there are differences in the age of weaning completion and duration: Early Neolithic groups weaned their infants at a later age and over a shorter amount of time. Differences may have affected infant morbidity and mortality, and female fecundity and inter\u2010birth intervals. Stable isotope values in older subadults (4\u201310 years, <jats:italic>n<\/jats:italic> = 26) do not differ from adults suggesting the absence of age\u2010based food allocation. 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