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Dentine incremental growth structures are complex, however, and current approaches that apply horizontal sectioning of demineralized tooth halves or quarters risk combining multiple growth layers and may include unwanted cementum or secondary dentine. They also require destruction of large parts of a tooth. Here, we present a less destructive and relatively straightforward protocol that reduces damage, increases temporal resolution, and improves the accuracy of age\u2010alignment between individuals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>We outline a protocol that includes the sampling of small (1\u2009mm diameter) cylindrical plug transects from a thin section, along with an age\u2010alignment scheme predicated on average growth rates for dentine areas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>The proposed protocol is readily applicable and more anatomically sensitive than horizontal slicing. Micro\u2010samples are smaller (in both length and depth), hence minimizing temporal overlap and avoid directions that may contravene growth pattern. They completely avoid areas where secondary and tertiary dentine or cementum can be deposited. Age\u2010alignment is improved by using growth ratios of anatomical tooth zones.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This method minimizes destruction, enables finer temporal resolution and facilitates data comparison. It can be readily combined with fluorescence imaging\u2010based or other pre\u2010screening methods of dentine collagen preservation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ajpa.24126","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T21:54:22Z","timestamp":1597182862000},"page":"776-783","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["A guide for an anatomically sensitive dentine microsampling and age\u2010alignment approach for human teeth isotopic sequences"],"prefix":"10.1002","volume":"173","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1264-2700","authenticated-orcid":false,"given":"Andrea","family":"Czermak","sequence":"first","affiliation":[{"name":"Research Laboratory for Archaeology and the History of Art, School of Archaeology University of Oxford  Oxford 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