{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:01:52Z","timestamp":1778846512824,"version":"3.51.4"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T00:00:00Z","timestamp":1325116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"Abstract<\/jats:title>Background:<\/jats:title>P\u2010glycoprotein (P\u2010gp) is a 170\u2010kDa transmembrane glycoprotein encoded by the MDR1 (ABCB1) gene and is constitutively expressed on lower airway epithelium. P\u2010gp has been shown to function as an immunomodulator regulating efflux of T\u2010helper 1\/T\u2010helper 2 (Th1\/Th2) cytokines from its host cell; however, its association with sinonasal inflammation has not been described. The purpose of this study is to determine the pattern and degree of epithelial P\u2010gp expression in chronic rhinosinusitis (CRS) with nasal polyposis (CRSwNP) or CRS without nasal polyposis (CRSsNP).<\/jats:p><\/jats:sec>Methods:<\/jats:title>Institutional Review Board (IRB)\u2010approved study utilizing sinus, septal, and inferior turbinate mucosa in patients with no disease, CRS, and CRSwNP (n = 4 each). Quantitative fluorescent immunohistochemistry (Q\u2010FIHC) was performed using an anti\u2010P\u2010gp antibody and a secondary fluorescein isothiocyanate (FITC)\u2010conjugated Fc specific fragment. Protein expression was quantified by calculating the epithelial to nonspecific background intensity ratio (4 images\/subsite). Scores less than 1 suggested negligible expression. Staining ratios between patient groups and subsites were compared using a 2\u2010tailed Student t<\/jats:italic> test.<\/jats:p><\/jats:sec>Results:<\/jats:title>Among the sinus mucosa, P\u2010gp expression in CRSwNP (1.570 \u00b1 0.354) was significantly greater than both CRS (1.224 \u00b1 0.248) and control (0.762 \u00b1 0.128) (p<\/jats:italic> < 0.001, p<\/jats:italic> = 0.002; respectively). CRS scores were significantly greater than control (p<\/jats:italic> = 0.002). Among the septal mucosa, there was no significant difference between CRSwNP (0.914 \u00b1 0.264), CRS (1.126 \u00b1 0.476), or control (0.966 \u00b1 0.327). Among the inferior turbinate mucosa, there was no significant difference between CRSwNP (1.047 \u00b1 0.157), CRS (1.099 \u00b1 0.362), or control (0.824 \u00b1 0.181).<\/jats:p><\/jats:sec>Conclusion:<\/jats:title>MDR1\/P\u2010gp is overexpressed in the epithelial layer of sinus mucosa in patients with both CRSwNP and CRS relative to other sinonasal subsites. Expression in healthy mucosa is negligible. Given its known immunomodulatory function this suggests that P\u2010gp may play a role in the pathogenesis or maintenance of chronic sinonasal inflammation. \u00a9 2011 ARS\u2010AAOA, LLC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21004","type":"journal-article","created":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T15:39:25Z","timestamp":1325173165000},"page":"122-125","source":"Crossref","is-referenced-by-count":31,"title":["Regional expression of epithelial MDR1\/P\u2010glycoprotein in chronic rhinosinusitis with and without nasal polyposis"],"prefix":"10.1002","volume":"2","author":[{"given":"Benjamin S.","family":"Bleier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,29]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"359","article-title":"Influence of the pro\u2010inflammatory cytokines on P\u2010glycoprotein expression and functionality","volume":"7","author":"Fernandez C","year":"2004","journal-title":"J Pharm Pharm Sci"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023230328687"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.10424"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008212"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/02770900902777767"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.06.726"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.04.006"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215540004800905"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.2500\/105065897781446603"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-005-5420-3"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2011.04325.x"}],"container-title":["International Forum of Allergy & Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Falr.21004","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.21004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T06:38:43Z","timestamp":1700548723000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/alr.21004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,29]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/alr.21004"],"URL":"https:\/\/doi.org\/10.1002\/alr.21004","archive":["Portico"],"relation":{},"ISSN":["2042-6976","2042-6984"],"issn-type":[{"value":"2042-6976","type":"print"},{"value":"2042-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,29]]}}}