{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T06:05:22Z","timestamp":1749189922493},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T00:00:00Z","timestamp":1352246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Nasal polyps in patients with cystic fibrosis (CF) are believed to be phenotypically different than polyps affecting non\u2010CF patients. The aim of this study was to investigate differences in cell cycle regulatory mechanisms between these 2 groups. In this prospective study at a tertiary care academic medical center, multiple techniques were used to confirm the upregulation of antiapoptotic Bcl\u20102 family proteins in CF polyps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nasal polyps were prospectively obtained from CF and non\u2010CF patients. The Sigma Panorama Protein Microarray for Cell Signaling was used to identify differences in protein expression between the 2 polyp groups. Western blot analysis confirmed altered expression of a subset of these proteins. Immunohistochemical staining was performed on archived tissue to further investigate B\u2010cell lymphoma 2 protein (Bcl\u20102) expression. Following review by a pathologist, slides were digitized using an Aperio\u2122 ScanScope XT system and staining intensity was quantified with the Positive Pixel Count algorithm. The mean staining intensity for each polyp group was compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The protein microarray suggested a greater than 2\u2010fold upregulation of Bcl\u2010xl in CF polyps relative to non\u2010CF polyps. Western blot analysis confirmed the upregulation in CF polyps of Bcl\u20102, a more commonly studied protein analog of Bcl\u2010xl. The CF polyp group was noted to have a higher quantitative intensity of immunohistochemical staining for Bcl\u20102 compared to the non\u2010CF group (<jats:italic>p<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Through multiple modalities of protein investigation, we have demonstrated an upregulation of Bcl\u20102 family proteins in CF polyps relative to polyps from non\u2010CF patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21107","type":"journal-article","created":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T17:46:34Z","timestamp":1352310394000},"page":"199-203","source":"Crossref","is-referenced-by-count":2,"title":["Upregulation of Bcl\u20102 in nasal polyps from patients with cystic fibrosis"],"prefix":"10.1002","volume":"3","author":[{"given":"Victor I.","family":"Scapa","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Colorado Denver  Aurora CO"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay R.","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Colorado Denver  Aurora CO"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd T.","family":"Kingdom","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Colorado Denver  Aurora CO"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-9725.2003.00061.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2005.08.010"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/019459988709700403"},{"key":"e_1_2_6_5_1","first-page":"1067","article-title":"Treatment and prognosis of nasal polyps in cystic fibrosis","volume":"136","author":"Stern RC","year":"1982","journal-title":"Am J Dis Child"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348948709600403"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2310\/7070.2001.21006"},{"key":"e_1_2_6_8_1","first-page":"445","article-title":"Differential pathology of nasal polyps in cystic fibrosis and atopy","volume":"40","author":"Oppenheimer E","year":"1979","journal-title":"Lab Invest"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1028"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6200"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2009.23.3314"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2006.02514.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(91)90171-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401305"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.2004.04418.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890160072013"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.50.11.923"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.045"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2006.20.2908"}],"container-title":["International Forum of Allergy &amp; Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Falr.21107","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.21107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T06:10:41Z","timestamp":1698646241000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/alr.21107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,7]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1002\/alr.21107"],"URL":"https:\/\/doi.org\/10.1002\/alr.21107","archive":["Portico"],"relation":{},"ISSN":["2042-6976","2042-6984"],"issn-type":[{"value":"2042-6976","type":"print"},{"value":"2042-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,7]]}}}