{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:06:40Z","timestamp":1758632800361},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T00:00:00Z","timestamp":1383523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>We previously reported that vascular endothelial cell growth factor (VEGF) is abundantly expressed by primary human nasal epithelial cells (PNECs) and functions to promote cell hyperplasia in polyposis. Therefore, we aimed to examine the full expression profile of other members of the VEGF gene family of ligands and receptors, which may play a role in cell growth and the development of chronic rhinosinusitis with nasal polyposis (CRSwNP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Messenger RNA (mRNA) and protein expression of VEGF genes, receptors, and co\u2010receptors was examined from cultured PNECs (n\u00a0= 4) and compared to that from primary human bronchial epithelial cells (PBECs; n\u00a0= 4) and the BEAS2B cell line (n\u00a0= 4) by real\u2010time polymerase chain reaction (PCR) and flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We report abundant expression of VEGFA, VEGFB, and VEGFC, detected by mRNA and flow cytometric analysis on PNECs. We herein report the novel finding that there is significant expression of VEGFR1, VEGFR2, VEGFR3, and both neuropilin co\u2010receptors, NP1 and NP2, at baseline conditions on PNECs. Lower airway PBECs and BEAS2B cells displayed similar patterns of expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PNECs express high constitutive levels of the VEGF gene family homolog of ligands and receptors. Expression of multiple VEGF ligand\u2010receptor combinations may function as redundant pathways to promote upper and lower airway epithelial cell growth during inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21244","type":"journal-article","created":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T17:26:41Z","timestamp":1383586001000},"page":"8-14","source":"Crossref","is-referenced-by-count":7,"title":["Constitutive expression of vascular endothelial cell growth factor (VEGF) gene family ligand and receptors on human upper and lower airway epithelial cells"],"prefix":"10.1002","volume":"4","author":[{"given":"Hyun Sil","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Medicine: Allergy and Clinical Immunology Johns Hopkins Asthma and Allergy Center Johns Hopkins Bayview Medical Center Johns Hopkins University School of Medicine  Baltimore MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medicine: Allergy and Clinical Immunology Johns Hopkins Asthma and Allergy Center Johns Hopkins Bayview Medical Center Johns Hopkins University School of Medicine  Baltimore MD"},{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery Johns Hopkins Asthma and Allergy Center Johns Hopkins Bayview Medical Center Johns Hopkins University School of Medicine  Baltimore MD"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.01.008"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200905-0740OC"},{"key":"e_1_2_6_4_1","first-page":"34","volume-title":"Proceedings of the 58th Annual Meeting of the American Rhinologic Society","author":"Kim J","year":"2012"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21129"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2007.01.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.12.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1013"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.45.32127"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.8.3336.bloodjournal8783336"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.11.2768"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.12.3829"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81402-6"},{"key":"e_1_2_6_14_1","unstructured":"LeeH MyersA KimJ.Involvement of VEGF in upper airway epithelial cell dysfunction in chronic rhinosinusitis with nasal polyposis subjects [Abstract]. Presented at the Keystone Symposium on Molecular and Cellular Biology. J1: Allergy and Asthma. 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