{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:05:17Z","timestamp":1770746717985,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T00:00:00Z","timestamp":1398988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Background<\/jats:title>Clinical allergy cross\u2010reactivity that is seen with related inhalant allergens or between unrelated inhalant allergens and foods in oral allergy syndrome (OAS) remains poorly understood. The goal of this study is to determine whether clinical cross\u2010reactivity can be identified from primary protein sequences in allergy epitopes and food proteins.<\/jats:p><\/jats:sec>Methods<\/jats:title>High\u2010throughput analysis was performed by assembling all known allergy epitopes within the Immune Epitope Database (IEDB; http:\/\/www.iedb.org<\/jats:ext-link>) for 5 common species from 5 inhalant allergen subclasses and comparing their protein sequences to each other, as well as to sequences of intact proteins from known cross\u2010reactive foods in the European Molecular Biology Laboratory\u2013European Bioinformatics Institute (EMBL\u2010EBI) protein database (http:\/\/www.uniprot.org<\/jats:ext-link>) that have been implicated in OAS. Computational methods were employed to allow for exact matching, gaps, and similar amino acids using multiple algorithms. A phylogenetic tree was created to determine evolutionary relationships between cross\u2010reactive epitopes in OAS.<\/jats:p><\/jats:sec>Results<\/jats:title>Twenty\u2010three common inhalant allergens had 4429 unique epitopes; the 19 foods implicated in OAS had 9497 protein sequences. The Basic Local Alignment Search Tool (BLAST) algorithm identified interclass and intraclass sequence similarities for the 5 inhalant allergy classes with high similarity for mites, grasses, and trees. Analysis of OAS proteins identified 104 matches to inhalant allergy epitopes that are known to cross\u2010react. The phylogenetic tree displayed relationships that mostly followed organism phylogeny.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Use of primary protein sequences was successful in explaining clinical allergy cross\u2010reactivity. Clinical correlation is needed for use of these epitopes as diagnostic or therapeutic entities for patients with cross\u2010reactive allergic disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21340","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T17:10:39Z","timestamp":1399050639000},"page":"559-564","source":"Crossref","is-referenced-by-count":11,"title":["Allergen cross\u2010reactivity in allergic rhinitis and oral\u2010allergy syndrome: a bioinformatic protein sequence analysis"],"prefix":"10.1002","volume":"4","author":[{"given":"Michael","family":"Platt","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Howell","sequence":"additional","affiliation":[{"name":"Bioinformatics Program Department of Engineering Boston University Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricky","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Bioinformatics Program Department of Engineering Boston University Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Dumont","sequence":"additional","affiliation":[{"name":"Division of Pulmonology Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1683"},{"key":"e_1_2_6_3_1","first-page":"58","article-title":"Oral allergy syndrome\u2014what's new","volume":"22","author":"Steinman H","year":"2009","journal-title":"Curr Allergy Clin Immunol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.10.026"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056006478.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2006.11.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76410-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.00994.x"},{"key":"e_1_2_6_9_1","unstructured":"Immune Epitope Database and Analysis Resource.http:\/\/www.iedb.org. Accessed April 14 2014."},{"key":"e_1_2_6_10_1","unstructured":"European Molecular Biology Laboratory\u2013European Bioinformatics Institute (EMBL\u2010EBI) protein database.http:\/\/www.uniprot.org. Accessed April 14 2014."},{"key":"e_1_2_6_11_1","unstructured":"National Center for Biotechnology Information (NCBI).BLAST Basic Local Alignment Search Tool.http:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi. Accessed April 14 2014."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0804-1035"},{"key":"e_1_2_6_13_1","volume-title":"Codex Principles and Guidelines on Foods Derived From Biotechnology","author":"Food and Agricultural Organization of the United Nations\/World Health Organization (FAO\/WHO).","year":"2003"},{"key":"e_1_2_6_14_1","unstructured":"National Center for Biotechnology Information (NCBI).Taxonomy.http:\/\/www.ncbi.nlm.nih.gov\/taxonomy?db=taxonomy. Accessed April 14 2014."},{"key":"e_1_2_6_15_1","unstructured":"Phylodendron Phylogenetic Tree Printer.http:\/\/iubio.bio.indiana.edu\/treeapp\/treeprint\u2010form.html. Accessed April 14 2014."},{"key":"e_1_2_6_16_1","first-page":"147","article-title":"Rhinitis: a dose of epidemiological reality","volume":"24","author":"Settipane RA","year":"2003","journal-title":"Allergy Asthma Proc"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(03)02008-6"},{"key":"e_1_2_6_18_1","first-page":"87","article-title":"Tree pollen allergens","volume":"21","author":"Swoboda I","year":"2008","journal-title":"Clin Allergy Immunol"},{"key":"e_1_2_6_19_1","first-page":"141","article-title":"Mite allergens: an overview","volume":"43","author":"Bessot JC","year":"2011","journal-title":"Eur Ann Allergy Clin Immunol"},{"key":"e_1_2_6_20_1","unstructured":"Structural Database of Allergenic Proteins (SDAP).https:\/\/fermi.utmb.edu\/SDAP. Accessed April 14 2014."}],"container-title":["International Forum of Allergy & Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Falr.21340","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.21340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T14:39:12Z","timestamp":1696603152000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/alr.21340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,2]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/alr.21340"],"URL":"https:\/\/doi.org\/10.1002\/alr.21340","archive":["Portico"],"relation":{},"ISSN":["2042-6976","2042-6984"],"issn-type":[{"value":"2042-6976","type":"print"},{"value":"2042-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,2]]}}}