{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:03:11Z","timestamp":1788580991638,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R15DE021194-01"],"award-info":[{"award-number":["1R15DE021194-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Topical treatments with nasal saline irrigation, topical steroid sprays, or corticosteroid rinses can improve sinonasal symptoms in chronic rhinosinusitis (CRS). However, the impact of these therapies on commensals (<jats:italic>Corynebacterium<\/jats:italic>) and on biofilm pathogens associated with CRS (<jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Pseudomonas<\/jats:italic>) is not well characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paired nasal and sinus swabs were collected endoscopically from 28 controls and 14 CRS patients with nasal polyposis (CRSwNP) who had not received systemic antibiotics or corticosteroids in the previous 8 weeks. Total DNA from swab eluents were extracted and analyzed by 16S rRNA gene\u2010based pyrosequencing. A total of 359,077 reads were obtained and classified taxonomically. The association of use of topical therapies with sinonasal microbiota composition was assessed by factor\/vector\u2010fitting. The proportional abundances of sinonasal bacteria between topical therapy users and nonusers were further compared by 2\u2010tailed Kolmogorov\u2010Smirnov test among controls and among CRSwNP participants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nasal saline irrigation, with or without added budesonide, was not associated with significantly distinct sinonasal microbiota composition or significantly decreased <jats:italic>Pseudomonas<\/jats:italic> or <jats:italic>S. aureus<\/jats:italic> abundances among either controls or CRSwNP participants. <jats:italic>Corynebacterium<\/jats:italic> was slightly lower in controls that reported using saline irrigation than those who did not. No significant association was found between nasal saline irrigation and the proportional abundances of <jats:italic>Pseudomonas<\/jats:italic>, <jats:italic>S. aureus<\/jats:italic>, and <jats:italic>Corynebacterium<\/jats:italic> in CRSwNP participants. However, male CRSwNP patients were noted to have significantly higher <jats:italic>Corynebacterium<\/jats:italic> proportional abundances than their female counterparts. The use of topical steroid sprays was associated with a distinct microbiota in control subjects, characterized by higher proportional abundances of <jats:italic>Dolosigranulum<\/jats:italic> and <jats:italic>Simonsiella<\/jats:italic> and a lower proportional abundance of <jats:italic>Campylobacter<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nasal saline irrigation is not associated with a distinct alteration in the proportional abundance of commensal bacteria or biofilm\u2010forming pathogens in CRSwNP patients. However, use of topical intranasal corticosteroid sprays in control subjects is associated with a distinct sinonasal microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21467","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T17:02:15Z","timestamp":1419872535000},"page":"185-190","source":"Crossref","is-referenced-by-count":44,"title":["Impact of saline irrigation and topical corticosteroids on the postsurgical sinonasal microbiota"],"prefix":"10.1002","volume":"5","author":[{"given":"Cindy M.","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Medicine Johns Hopkins University  Baltimore MD"},{"name":"Center for Microbial Genetics and Genomics Northern Arizona University  Flagstaff AZ"},{"name":"Division of Pathogen Genomics Translational Genomics Research Institute  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Kohanski","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins School of Medicine  Baltimore MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Mendiola","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Johns Hopkins School of Medicine  Baltimore MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Soldanova","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics Northern Arizona University  Flagstaff AZ"},{"name":"Division of Pathogen Genomics Translational Genomics Research Institute  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Dwan","sequence":"additional","affiliation":[{"name":"Center for Microbial Genetics and Genomics Northern Arizona University  Flagstaff AZ"},{"name":"Division of 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