{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T07:54:45Z","timestamp":1773042885189,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T00:00:00Z","timestamp":1447718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Patients with chronic rhinosinusitis with nasal polyps (CRSwNP) have deficiencies in circulating and sinonasal levels of the inactive form of vitamin D3, 25\u2010hydroxycholecalciferol (25VD3). Moreover, CRSwNP patients have reduced epithelial cell\u2013specific expression of 1\u03b1\u2010hydroxylase; the enzyme responsible for the conversion of 25VD3 to its metabolically active form, 1\u03b1,25\u2010dihydroxyvitamin D3 (1,25VD3). The objective of this work was to determine the impact of sinonasal 1\u03b1\u2010hydroxylase levels combined from all cellular sources on subjective disease severity and to identify variables influencing its expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood and sinus tissue explants were collected at the time of surgery from control, chronic rhinosinusitis without nasal polyps (CRSsNP), CRSwNP, and allergic fungal rhinosinusitis (AFRS) patients. 1\u03b1\u2010Hydroxylase was measured by immunostaining with flow cytometric analysis. Subjective disease severity was measured by the 22\u2010item Sino\u2010Nasal Outcomes Test (SNOT\u201022). 1,25VD3 and 25VD3 were measured by enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with CRSwNP or AFRS have reduced 1\u03b1\u2010hydroxylase and 1,25VD3 compared to controls or CRSsNP. Circulating 1,25VD3 levels were the same among all groups. No differences in sinonasal 1\u03b1\u2010hydroxylase or 1,25VD3 were found between CRSwNP and AFRS. Gender, age, race, atopy, and systemic 25VD3 had no impact on sinonasal 1\u03b1\u2010hydroxylase levels in any group. However, CRSwNP patients with asthma had higher 1\u03b1\u2010hydroxylase than those without asthma. Total 1\u03b1\u2010hydroxylase levels inversely correlated with SNOT\u201022 in CRSwNP, but not CRSsNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Patients with CRSwNP and AFRS both have reduced sinonasal 1\u03b1\u2010hydroxylase and 1,25VD3 compared to controls or CRSsNP. Reductions in intracellular 1\u03b1\u2010hydroxylase combined from all sinonasal cell types were associated with more severe subjective disease severity in CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21576","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T13:59:01Z","timestamp":1447768741000},"page":"58-65","source":"Crossref","is-referenced-by-count":27,"title":["Reduced sinonasal levels of 1\u03b1\u2010hydroxylase are associated with worse quality of life in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1002","volume":"6","author":[{"given":"Rodney J.","family":"Schlosser","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery, Medical University of South Carolina  Charleston SC"},{"name":"Ralph H. 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