{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T18:08:37Z","timestamp":1740161317656,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundacao de Amparo a Pesquisa do Estado de Sao Paulo"},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cientifico e Tecnologico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) are involved in allergic processes and play a role in adenotonsillar hyperplasia (AH). Clinically, only CysLTR1 may be blocked by montelukast. Our objective was to compare the expression of CysLTR1 and CysLTR2 in the B and T cells of hyperplasic tonsils of sensitized (SE) and control (NS) snoring children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty children, 5 to 10 years of age, referred for adenotonsillectomy, were divided into SE and NS groups, according to their responses to the skin\u2010prick test. Cells from the removed tissues were stained for CysLTR1, CysLTR2, CD19, and CD3 and counted via flow cytometry. messenger RNA (mRNA) expression of the CysLTRs genes was measured real\u2010time quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The SE group showed reduced expression of the small CD3+\/CysLTR1+ lymphocytes (4.6 \u00b1 2.2 vs 6.5 \u00b1 5.0; <jats:italic>p<\/jats:italic> = 0.04). Regarding the large lymphocytes, the SE group showed lower expression of CD3+\/CysLTR1+ (40.9 \u00b1 14.5 vs 47.6 \u00b1 11.7; <jats:italic>p<\/jats:italic> = 0.05), CD19+\/CysLTR1+ (44.6 \u00b1 16.9 vs 54.1 \u00b1 12.4; <jats:italic>p<\/jats:italic> = 0.01), and CD19+\/CysLTR2+ (55.3 \u00b1 11.3 vs 61.5 \u00b1 12.6; <jats:italic>p<\/jats:italic> = 0.05) lymphocytes. Considering the total number of lymphocytes, the SE group had fewer CD3+\/CysLTR1+ lymphocytes (11.1 \u00b1 5.5 vs 13.7 \u00b1 6.2; <jats:italic>p<\/jats:italic> = 0.04). All other cell populations exhibited reduced expression in the SE group without statistical significance. The expression of CysLTR2 was significantly higher (<jats:italic>p<\/jats:italic> &lt; 0.05) than CysLTR1 in most studied cell populations. The mRNA expression did not show significant differences between the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of CysLTR is higher in the lymphocytes of the NS children, and CysLTR2 shows greater expression than CysLTR1 Respiratory allergies do not appear to be a stimulus for AH occurrence. Newer drugs capable of blocking both CysLTRs warrant further study.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.21798","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T10:29:10Z","timestamp":1464172150000},"page":"1151-1158","source":"Crossref","is-referenced-by-count":6,"title":["Expression of cysteinyl leukotriene receptor 1 and 2 (CysLTR1 and CysLTR2) in the lymphocytes of hyperplastic tonsils: comparison between allergic and nonallergic snoring children"],"prefix":"10.1002","volume":"6","author":[{"given":"Bruno Peres","family":"Paulucci","sequence":"first","affiliation":[{"name":"Department of Otolaryngology of Hospital das Clinicas University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Hematology of Hospital das Clinicas University of Sao Paulo  Sao Paulo 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