{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:50:15Z","timestamp":1790974215708,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T00:00:00Z","timestamp":1483315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","award":["UL1 TR000135"],"award-info":[{"award-number":["UL1 TR000135"]}],"id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Endotyping chronic rhinosinusitis (CRS) through simplified cytokine assays may help direct individualized therapy such as corticosteroids, antibiotics, or biologics. We performed an unsupervised network analysis to endotype CRS and control subjects using a commercially available cytokine\u2010chemokine immunoassay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A 41\u2010plex cytokine\u2010chemokine array along with major basic protein (MBP) assay was performed on sinonasal surgical tissue of 32 adults. Subjects were defined as non\u2010CRS controls (n = 6), CRS with nasal polyps (CRSwNP; n = 13), and CRS without nasal polyps (CRSsNP; n = 13). Unsupervised network modeling was performed to reveal association cytokine\u2010chemokine (\u201canalyte\u201d) clusters and \u201csubject\u201d groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Network mapping and unsupervised clustering revealed 3 analyte clusters and 3 subject groups. Analyte cluster\u20101 was composed of T helper 1 (Th1)\/Th17 type markers, analyte cluster\u20102 Th2 markers, and analyte cluster\u20103 chemokines (CC) and growth factors (GF). Subject group\u20101 was devoid of CRSwNP, had fewer asthmatics, and was associated most strongly with analyte cluster\u20103 (CC\/GF) (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Subject group\u20102 was characterized with the most asthmatics (86%) and CRSwNP (100%) patients, and was associated with analyte cluster\u20102 (Th2;\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Subject group\u20103 was associated with both analyte cluster\u20101 (Th1\/Th17) and analyte cluster\u20103 (CC\/GF) (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001), and had the highest proportion of CRSsNP patients (62.5%). Tissue levels of MBP, eosinophilia, and computed tomography (CT) scores were significantly higher in subject group\u20102 vs other groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2264 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>An unbiased network\u2010mapping approach using a commercially available immunoassay kit reveals 3 distinct tissue cytokine\u2010chemokine signatures that endotype CRS patients and controls. These signatures are prominent even in a limited number of patients, and may help formulate individualized therapy and optimize outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.21904","type":"journal-article","created":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T04:46:50Z","timestamp":1483332410000},"page":"373-379","source":"Crossref","is-referenced-by-count":38,"title":["Unsupervised network mapping of commercially available immunoassay yields three distinct chronic rhinosinusitis endotypes"],"prefix":"10.1002","volume":"7","author":[{"given":"Rohit","family":"Divekar","sequence":"first","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic in Rochester  Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Rank","sequence":"additional","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic in Arizona  Scottsdale AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Squillace","sequence":"additional","affiliation":[{"name":"Allergic Diseases Research Laboratory Mayo Clinic  Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohito","family":"Kita","sequence":"additional","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic in Rochester  Rochester MN"},{"name":"Allergic Diseases Research Laboratory Mayo Clinic  Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devyani","family":"Lal","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Mayo Clinic in Arizona  Phoenix AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599815572097"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2016.05.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.3.320"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.08.004"},{"issue":"256","key":"e_1_2_6_6_1","first-page":"1","article-title":"Summary health statistics for U.S. adults: National Health Interview Survey","author":"Schiller JS","journal-title":"Vital Health Stat 10"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.08.037"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.11.037"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.02.036"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.017"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.12.1324"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.03.011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4486.2009.01995.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-59989770005-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.06.726"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21032"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.09.006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098679"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2016.04.012"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.19330"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12463"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/194589240501900102"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2011.25.3535"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201412-2278OC"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-7-25"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21082"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000133"}],"container-title":["International Forum of Allergy &amp; 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