{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T20:42:29Z","timestamp":1793392949499,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T00:00:00Z","timestamp":1494201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Mayo Clinic Center for Clinical and Translational Science","award":["UL1 TR000135"],"award-info":[{"award-number":["UL1 TR000135"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The role of microbiota in sinonasal inflammation can be further understood by targeted sampling of healthy and diseased subjects. We compared the microbiota of the middle meatus (MM) and inferior meatus (IM) in healthy, allergic rhinitis (AR), and chronic rhinosinusitis (CRS) subjects to characterize intrasubject, intersubject, and intergroup differences.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Subjects were recruited in the office, and characterized into healthy, AR, and CRS groups. Endoscopically\u2010guided swab samples were obtained from the MM and IM bilaterally. Bacterial microbiota were characterized by sequencing the V3\u2010V4 region of the 16S ribosomal RNA (rRNA) gene.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intersubject microbiome analyses were conducted in 65 subjects: 8 healthy, 11 AR, and 46 CRS (25 CRS with nasal polyps [CRSwNP]; 21 CRS without nasal polyps [CRSsNP]). Intrasubject analyses were conducted for 48 individuals (4 controls, 11 AR, 8 CRSwNP, and 15 CRSwNP). There was considerable intersubject microbiota variability, but intrasubject profiles were similar (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.001, nonparametric\n                      <jats:italic>t<\/jats:italic>\n                      test). Intrasubject bacterial diversity was significantly reduced in MM of CRSsNP subjects compared to IM samples (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.022, nonparametric\n                      <jats:italic>t<\/jats:italic>\n                      test). CRSsNP MM samples were enriched in\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Haemophilus<\/jats:italic>\n                      , and\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      spp. but exhibited loss of diversity compared to healthy, CRSwNP, and AR subject\u2010samples (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05; nonparametric\n                      <jats:italic>t<\/jats:italic>\n                      test). CRSwNP patients were enriched in\n                      <jats:italic>Staphylococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Alloiococcus<\/jats:italic>\n                      , and\n                      <jats:italic>Corynebacterium<\/jats:italic>\n                      spp.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study presents the sinonasal microbiome profile in one of the larger populations of non\u2010CRS and CRS subjects, and is the first office\u2010based cohort in the literature. In contrast to healthy, AR, and CRSwNP subjects, CRSsNP MM samples exhibited decreased microbiome diversity and anaerobic enrichment. CRSsNP MM samples had reduced diversity compared to same\u2010subject IM samples, a novel finding.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.21934","type":"journal-article","created":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T06:56:43Z","timestamp":1494226603000},"page":"561-569","source":"Crossref","is-referenced-by-count":111,"title":["Mapping and comparing bacterial microbiota in the sinonasal cavity of healthy, allergic rhinitis, and chronic rhinosinusitis subjects"],"prefix":"10.1002","volume":"7","author":[{"given":"Devyani","family":"Lal","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Mayo Clinic in Arizona  Phoenix AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Keim","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"},{"name":"Pathogen and Microbiome Institute Northern Arizona University  Flagstaff AZ"},{"name":"Department of Biological Sciences Northern Arizona University  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josie","family":"Delisle","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget","family":"Barker","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"},{"name":"Pathogen and Microbiome Institute Northern Arizona University  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Rank","sequence":"additional","affiliation":[{"name":"Division of Allergy, Asthma, and Clinical Immunology Mayo Clinic in Arizona  Scottsdale AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Chia","sequence":"additional","affiliation":[{"name":"Center for Individualized Medicine, Microbiome Program Mayo Clinic  Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Schupp","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Gillece","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily K.","family":"Cope","sequence":"additional","affiliation":[{"name":"Translational Genomics Research Institute  Flagstaff AZ"},{"name":"Pathogen and Microbiome Institute Northern Arizona University  Flagstaff AZ"},{"name":"Department of Biological Sciences Northern Arizona University  Flagstaff AZ"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.08.037"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0194599815572097"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085507"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2016.30.4278"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21600"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00134"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003783"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.02.008"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21871"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(92)90161-T"},{"key":"e_1_2_6_12_1","first-page":"171","article-title":"Significance of osteomeatal complex obstruction","volume":"39","author":"Chandra RK","year":"2010","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123216"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.06.726"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348949510410s02"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-4486.2009.01995.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21195"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks808"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126626"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.12.001"},{"key":"e_1_2_6_21_1","unstructured":"St JohnJ.SeqPrep tool for stripping adaptors and\/or merging paired reads with overlap into single reads.https:\/\/github.com\/jstjohn\/SeqPrep. 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