{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:01:46Z","timestamp":1790622106126,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T00:00:00Z","timestamp":1515974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University Hospital of Clermont-Ferrand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common disease with an uncertain pathophysiology. It is characterized by polyps rich in eosinophils, with an activation status already investigated at the tissue level. In a group of CRSwNP patients, we assessed the activation status of circulating eosinophils in the blood before migration into tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirteen patients with CRSwNP and 16 healthy volunteers were enrolled. Several biologic parameters were studied: blood count of eosinophils; plasma eosinophil cationic protein; oxidative metabolism by chemiluminescence at baseline or when activated by phorbol 12\u2010myristate 13\u2010acetate or platelet\u2010activating factor, with or without interleukin\u20105 (IL\u20105); percent of granulosar cells; and mean fluorescence intensity (MFI) by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean number of eosinophils was significantly higher in patients with CRSwNP, whose eosinophils showed increased oxidative metabolism in the basal or activated state significantly decreasing in the presence of IL\u20105. There was also a higher percentage of CD49d<jats:sup>+<\/jats:sup>, CD25<jats:sup>+<\/jats:sup>, and CCR3<jats:sup>+<\/jats:sup>cells in patients, and a nonsignificant decrease in descending order in MFI between the control group, patients with normal eosinophil levels, patients with hypereosinophilia, and patients with aspirin\u2010exacerbated respiratory disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates a priming state of circulating eosinophils in CRSwNP patients when compared with healthy controls, as evidenced by the extent of oxidative metabolism, with increased sensitivity to IL\u20105 and by the observed variations of percent and MFI of CD49d, CCR3, and CD25. This priming is thus found at the peripheral level and occurs before the migration of eosinophils to polyps, reflecting the systemic and not just local nature of abnormalities in CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22079","type":"journal-article","created":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T14:12:54Z","timestamp":1516025574000},"page":"584-591","source":"Crossref","is-referenced-by-count":10,"title":["Activation state of circulating eosinophils in nasal polyposis"],"prefix":"10.1002","volume":"8","author":[{"given":"Vincent","family":"Dupuch","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery University Hospital Clermont\u2010Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlette","family":"Tridon","sequence":"additional","affiliation":[{"name":"Department of Immunology University Hospital Clermont\u2010Ferrand France"},{"name":"Universit\u00e9 Clermont Auvergne, INRA, 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