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However, it is unclear if PBML GC receptor isoforms are expressed differentially and are associated with worsened disease severity in chronic rhinosinusitis (CRS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PBMLs were isolated from control (n = 8), CRS without nasal polyps (CRSsNP) (n = 8), atopic CRS with nasal polyps (CRSwNP) (n = 8), non\u2010atopic CRSwNP (n = 8), and allergic fungal rhinosinusitis (AFRS) (n = 8) patients. Demographics, atopic status, asthmatic status, 22\u2010item Sino\u2010Nasal Outcome Test (SNOT\u201022) scores, Lund\u2010Kennedy nasal endoscopy scores, Lund\u2010Mackay sinus computed tomography (CT) scores, Kennedy Osteitis scores, and GC utilization 6 months postoperatively were collected. Intracellular immunostaining was then performed for functional GC receptor \u03b1 (GCR\u03b1) and nonfunctional GC receptor \u03b2 (GCR\u03b2), followed by flow cytometry analysis of geometric mean fluorescent intensity (MFI) and the percentage of cells expressing each GC receptor isoform.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to controls, each CRS subtype had decreased PBML GCR\u03b1 and GCR\u03b1:GCR\u03b2 MFI expression, but no difference in GCR\u03b2 expression. Decreasing PBML GCR\u03b1 in AFRS was associated with increasing Lund\u2010Mackay sinus CT scores (<jats:italic>r<\/jats:italic> = \u22120.880, <jats:italic>p<\/jats:italic> =0.004). No significant associations were found between GC receptor isoform expression and other clinical measures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CRS patients have reduced functional PBML GCR\u03b1 expression and decreased GCR\u03b1:GCR\u03b2 compared to controls. Reductions in GCR\u03b1 in AFRS are associated with worsening Lund\u2010Mackay sinus CT scores. The clinical implications of decreased functional GC receptor expression merits further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22120","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T19:15:13Z","timestamp":1525288513000},"page":"913-921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Glucocorticoid receptor isoform expression in peripheral blood mononuclear leukocytes of patients with chronic rhinosinusitis"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8196-6218","authenticated-orcid":false,"given":"Robert J.","family":"Taylor","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Medical University of South Carolina  Charleston SC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney J.","family":"Schlosser","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Medical University of South Carolina  Charleston SC"},{"name":"Ralph H. 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