{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:16:09Z","timestamp":1789496169208,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Much of the literature examining chronic rhinosinusitis with nasal polyps (CRSwNP) immunopathology has been predicated on messenger RNA (mRNA) analysis with the assumption that transcriptional changes would reflect end\u2010effector protein expression. The purpose of this study was to test this hypothesis using matched transcriptomic and proteomic data sets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Matched tissue proteomic and transcriptomic arrays were quantified in CRSwNP polyp tissue and control inferior turbinate tissue (n = 10\/group). Mucus samples were additionally collected in 6 subjects from each group. Proteins were grouped into functional categories by bioinformatics and differential expression analyses. Log\u2010log regression and Pearson correlations were performed to determine the level of agreement between data sets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 1310 proteins examined, 393 were significantly differentially expressed in CRSwNP. On regression analysis, differences in protein expression were poorly predicted by differences in mRNA expression (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      = 0.020,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Several genes canonically thought to be overexpressed in CRSwNP, including IL\u20105, IL\u201013, TSLP, CCL13, and CCL26, showed substantial increases in mRNA transcription, but had minimally or unchanged protein expression. Others, including IgE, periostin, CCL18, and CST1\/2, were increased at both the transcriptomic and proteomic levels. Among differentially regulated proteins, tissue and mucus protein levels showed weak correlation (\n                      <jats:italic>r<\/jats:italic>\n                      = 0.26,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Proteomic analysis in CRSwNP has revealed novel disease\u2010associated proteins and pathways, yet correlates poorly with transcriptomic data. The increasing availability of proteomic arrays opens the door to new potential explanatory mechanisms in CRSwNP and suggests that mRNA based studies should be validated with protein analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.22315","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T05:52:32Z","timestamp":1550469152000},"page":"776-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Translating transcription: proteomics in chronic rhinosinusitis with nasal polyps reveals significant discordance with messenger RNA expression"],"prefix":"10.1002","volume":"9","author":[{"given":"Alan D.","family":"Workman","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Massachusetts Eye and Ear Infirmary  Boston MA"},{"name":"Harvard Medical School  Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5480-415X","authenticated-orcid":false,"given":"Angela L.","family":"Nocera","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Massachusetts Eye and Ear Infirmary  Boston MA"},{"name":"Harvard Medical School  Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarina K.","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Massachusetts Eye and Ear Infirmary  Boston MA"},{"name":"Harvard Medical School  Boston MA"},{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery Friedrich\u2010Alexander University Erlangen\u2010N\u00fcrnberg (FAU)  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan H.","family":"Otu","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering University of Nebraska\u2010Lincoln  Lincoln NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Towia A.","family":"Libermann","sequence":"additional","affiliation":[{"name":"Harvard Medical School  Boston MA"},{"name":"Department of Medicine Beth Israel Deaconess Medical Center (BIDMC)  Boston MA"},{"name":"Division of Interdisciplinary Medicine and Biotechnology Beth Israel Deaconess Medical Center (BIDMC)  Boston MA"},{"name":"BIDMC Genomics, Proteomics, Bioinformatics and Systems Biology Center Beth Israel Deaconess Medical Center (BIDMC)  Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin S.","family":"Bleier","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Massachusetts Eye and Ear Infirmary  Boston MA"},{"name":"Harvard Medical School  Boston 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