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Numerous techniques for the study of ex\u2010vivo samples exist, with specific applications, strengths, and weaknesses associated with each of them. In this compendium we summarize the available methods for collection of primary human samples and incorporate expert discussion of the pros, cons, and applications associated with each technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An expert panel containing members of the American Rhinologic Society's Research and Grants Committee compiled this educational reference. Rationale for use and the potential advantages and disadvantages are discussed. Research protocols and key references are enumerated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sampling of the nasal cavity and paranasal sinuses can be achieved through a number of methods. Nonspecific sinonasal secretions may be collected via forced exhalation, nasal lavage, and nasal spray aspiration. Targeted collection of sinonasal secretions may be achieved via endoscopic placement of absorbent matrices. Nasal cytology or collection of superficial epithelium may be completed via brushing or scraping of endonasal structures. Collection of mucosal biopsies may be completed via sinonasal explant or full\u2010thickness biopsy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Multiple sampling techniques are available to collect biologic samples from the sinonasal cavity. These techniques differ in their ease of application, reproducibility, sample yield, and utility for different sinonasal pathologies or research goals. An appreciation of the benefits and drawbacks of each approach will allow investigators to select the techniques most appropriate for achieving research objectives.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22510","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T09:00:50Z","timestamp":1576573250000},"page":"303-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Sample collection for laboratory\u2010based study of the nasal airway and sinuses: a research compendium"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6558-4559","authenticated-orcid":false,"given":"Conner J.","family":"Massey","sequence":"first","affiliation":[{"name":"Department of Otolaryngology University of Colorado School of Medicine  Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Diaz Del Valle","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care University of Colorado School of Medicine  Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2489-0514","authenticated-orcid":false,"given":"Waleed M.","family":"Abuzeid","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Albert Einstein College of Medicine\/Montefiore Medical Center  Bronx NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5907-3421","authenticated-orcid":false,"given":"Joshua M.","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2012Head and Neck Surgery \u00a0Emory\u00a0University School of Medicine  Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarina","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Massachusetts Eye and Ear Infirmary Harvard Medical School  Boston MA"},{"name":"Department of Otolaryngology\/Head and Neck Surgery University of Erlangen\u2010Nuremberg  Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrina G.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Miller School of Medicine, University of Miami  Miami FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie S.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2012Head and Neck Surgery Northwestern University Feinberg School of Medicine  Chicago IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0783-8861","authenticated-orcid":false,"given":"Benjamin S.","family":"Bleier","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Massachusetts Eye and Ear Infirmary Harvard Medical School  Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2748-0705","authenticated-orcid":false,"given":"Vijay R.","family":"Ramakrishnan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology University of Colorado School of Medicine  Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.11.025"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0325OC"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.045"},{"key":"e_1_2_9_6_1","first-page":"293","volume-title":"Current Topics in Membranes. 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