{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:48:18Z","timestamp":1792097298210,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630023"],"award-info":[{"award-number":["81630023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570894"],"award-info":[{"award-number":["81570894"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970852"],"award-info":[{"award-number":["81970852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7172054"],"award-info":[{"award-number":["7172054"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase produces reactive oxygen species (ROS) involved in oxidative stress and signal transduction. Recent studies have suggested that NADPH oxidase is associated with the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). The aim of this study was to detect the expression of NADPH oxidase subunits and 4\u2010hydroxynonenal (4\u2010HNE) in nasal polyp tissue and normal nasal mucosa, in order to explore the possible role played by NADPH oxidase in the pathogenesis of CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirteen patients with CRSwNP and 9 normal control subjects were selected to participate in this study, in which we evaluated the expression of different NADPH oxidase subunits (gp91<jats:sup>phox<\/jats:sup>, p67<jats:sup>phox<\/jats:sup>, p47<jats:sup>phox<\/jats:sup>, and p22<jats:sup>phox<\/jats:sup>) in nasal polyp (NP) tissue and control mucosa by Western blotting and real\u2010time polymerase chain reaction (PCR). Immunohistochemistry and immunofluorescence staining were used to detect expression of the p67<jats:sup>phox<\/jats:sup> subunit and 4\u2010HNE in NP tissue and normal nasal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Western blot and real\u2010time PCR results showed that p67<jats:sup>phox<\/jats:sup> expression was significantly increased in NP tissue when compared with its expression in control mucosa (<jats:italic>p<\/jats:italic> = 0.004). p67<jats:sup>phox<\/jats:sup> was expressed in the eosinophils and neutrophils found in NP tissue, but not in the macrophages. Additionally, the levels of 4\u2010HNE expression were also significantly increased in NP tissue when compared with control mucosa (<jats:italic>p<\/jats:italic> = 0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The levels of p67<jats:sup>phox<\/jats:sup> messenger RNA (mRNA) and protein as well as 4\u2010HNE were both upregulated in NP tissue, suggesting that p67<jats:sup>phox<\/jats:sup> and oxidative stress play roles in the pathogenesis of CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22530","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T11:40:53Z","timestamp":1581594053000},"page":"646-655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Expression of nicotinamide adenine dinucleotide phosphate oxidase in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1002","volume":"10","author":[{"given":"Kaili","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Illinois at Chicago  Chicago IL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dachuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Harbin Medical University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangdong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0910-9884","authenticated-orcid":false,"given":"Luo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Department of Allergy Beijing TongRen Hospital Key Laboratory of Otolaryngology Head and Neck Surgery Ministry of Education Capital Medical University  Beijing China"},{"name":"Beijing Key Laboratory of Nasal Diseases Beijing Institute of Otolaryngology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhino12.000"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.201005-036RN"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3898"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21133"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200407000-00013"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01228.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.07.046"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-63245-2_8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3403"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.243972"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-8956-y"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2009.23.3340"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-011-0190-z"},{"key":"e_1_2_6_15_1","first-page":"S28","article-title":"Tissue distribution and putative physiological function of NOX family NADPH oxidases","volume":"57","author":"Krause KH","year":"2004","journal-title":"Jpn J Infect Dis"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.07.023"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00044.2005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.23787"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0194-59989770005-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2014.08.002"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-268"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2014.89"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12951"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.07.049"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5608"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.01.046"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-016-0403-y"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2010.2.3.183"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2013.04.002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2012.01294.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.10.003"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0252"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800469"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12944"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.10.497"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00101.2016"},{"key":"e_1_2_6_37_1","first-page":"391","article-title":"DNA damage caused by lipid peroxidation products","volume":"8","author":"\u0141uczaj W","year":"2003","journal-title":"Cell Mol Biol Lett"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.2110101"}],"container-title":["International Forum of Allergy &amp; Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Falr.22530","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.22530","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/alr.22530","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.22530","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T12:44:04Z","timestamp":1694004244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/alr.22530"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,13]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/alr.22530"],"URL":"https:\/\/doi.org\/10.1002\/alr.22530","archive":["Portico"],"relation":{},"ISSN":["2042-6976","2042-6984"],"issn-type":[{"value":"2042-6976","type":"print"},{"value":"2042-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,13]]},"assertion":[{"value":"2019-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}