{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:58:31Z","timestamp":1790697511238,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002462","name":"Chungnam National University","doi-asserted-by":"publisher","award":["2017\u20101919\u201001"],"award-info":[{"award-number":["2017\u20101919\u201001"]}],"id":[{"id":"10.13039\/501100002462","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017M3A9G5083517)."],"award-info":[{"award-number":["2017M3A9G5083517)."]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Povidone\u2010iodine (PVP\u2010I) is well known as an antiseptic and exhibits extensive activity against various pathogens. However, due to its uniquely unpleasant nature, it cannot be used locally to deactivate various sinonasal pathogens. Therefore, we developed a PVP\u2010I composite that blocks the unpleasant odor of PVP\u2010I for use as a local antiseptic in the sinonasal cavity and evaluated its effect on bacterial biofilm's formation and elimination in <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MTT, lactate dehydrogenase, and live\/dead staining assay were performed to examine the cellular toxicity of PVP\u2010I composites on the primary human nasal epithelial and RPMI 2650 cells. Crystal violet assay was performed to quantify bacterial biofilm after treating with various agents, including PVP\u2010I and antibiotics. Hematoxylin\u2010and\u2010eosin staining, live\/dead staining assay, and scanning electron microscopy were conducted to evaluate the effect of PVP\u2010I on biofilm formation in a mice biofilm model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>It was observed that the PVP\u2010I composite did not have any significant toxic effect on the nasal epithelial cells. Furthermore, the PVP\u2010I composite effectively inhibited the formation of bacterial biomass within a dose\u2010dependent manner after 48 hours of incubation with <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>. In mice, it effectively eliminated biofilm from the mucosa of the nasal cavity and maxillary sinus at the tested concentrations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study indicate that the PVP\u2010I composite is a promising compound that could be used locally to prevent the formation of biofilms and to eliminate them from the sinonasal cavity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22568","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T19:52:20Z","timestamp":1591041140000},"page":"884-892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Effects of povidone\u2010iodine composite on the elimination of bacterial biofilm"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9510-0909","authenticated-orcid":false,"given":"Leonita 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