{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:27:58Z","timestamp":1786976878279,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T00:00:00Z","timestamp":1595894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["5P30DK072482\u201005"],"award-info":[{"award-number":["5P30DK072482\u201005"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K08AI146220"],"award-info":[{"award-number":["K08AI146220"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>We recently developed a ciprofloxacin and azithromycin sinus stent (CASS) to target recalcitrant infections in chronic rhinosinusitis (CRS). The objective of this study was to evaluate the anti\u2010inflammatory activity of azithromycin released from the CASS and assess the impact on the integrity and function of primary human sinonasal epithelial cells (HSNECs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      lipopolysaccharide (LPS)\u2010stimulated HSNECs were treated with azithromycin and\/or ciprofloxacin at concentrations attainable from CASS release. Interleukin\u20108 (IL\u20108) secretion was quantified by enzyme\u2010linked immunosorbent assay (ELISA). Epithelial integrity (transepithelial resistance [TEER], paracellular permeability [fluorescein isothiocyanate\u2010labeled dextran], lactate dehydrogenase [LDH] assays) and function (ciliary beat frequency [CBF]) were also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Azithromycin significantly reduced secreted IL\u20108 from\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      LPS\u2010stimulated HSNECs at all concentrations tested (mean \u00b1 standard deviation; control = 5.77 \u00b1 0.39 ng\/mL, azithromycin [6 \u03bcg\/mL] = 4.58 \u00b1 0.40 ng\/mL, azithromycin [60 \u00b5g\/mL] = 4.31 \u00b1 0.06, azithromycin [180 \u00b5g\/mL] = 4.27 \u00b1 0.26 ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Co\u2010incubation with azithromycin (6 \u00b5g\/mL) and ciprofloxacin (2.4 \u00b5g\/mL) in LPS\u2010stimulated HSNECs also displayed a significant reduction in secreted IL\u20108 when compared to\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      LPS alone (co\u2010treatment = 4.61 \u00b1 0.29 ng\/mL,\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      LPS = 7.35 \u00b1 0.89 ng\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.01). The drugs did not negatively impact TEER, paracellular permeability, LDH release, or CBF, indicating retention of cell integrity and function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Azithromycin decreased\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      LPS IL\u20108 production in HSNECs at drug concentrations attainable with sustained release of azithromycin from the CASS. In addition to antibacterial activity, anti\u2010inflammatory properties of the CASS should provide further benefit for patients with recalcitrant CRS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.22656","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T23:24:31Z","timestamp":1595978671000},"page":"136-143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Azithromycin and ciprofloxacin inhibit interleukin\u20108 secretion without disrupting human sinonasal epithelial integrity in vitro"],"prefix":"10.1002","volume":"11","author":[{"given":"Dong\u2010Jin","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Alabama at Birmingham  Birmingham AL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harrison 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