{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:42:26Z","timestamp":1778042546892,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T00:00:00Z","timestamp":1613865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The precise mechanisms underlying pathogenesis of different subtypes of chronic rhinosinusitis with nasal polyps (CRSwNP) are still unclear. Emerging evidence indicates that microRNAs may play a role in the pathogenesis of CRSwNP. This study aimed to identify the dysregulated microRNA\u2012messenger RNA (miRNA\u2010mRNA) regulatory networks in eosinophilic (E) and non\u2010eosinophilic (non\u2010E) CRSwNP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole\u2010transcriptome sequencing was performed on nasal tissues of patients with ECRSwNP and non\u2010ECRSwNP, and control subjects. An integrated analysis of miRNA and mRNA expression was conducted to identify key mRNAs and miRNAs involved in the pathogenesis of ECRSwNP and non\u2010ECRSwNP. The miRNAs of interest and their target genes were validated using quantitative real\u2010time polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A group of differentially expressed mRNAs (DE\u2010mRNAs) and miRNAs (DE\u2010miRs) were identified in ECRSwNP patients vs control subjects, non\u2010ECRSwNP patients vs control subjects, and non\u2010ECRSwNP vs ECRSwNP patients, respectively. Pathway enrichment analysis showed distinct immune and inflammatory functions associated with DE\u2010mRNAs and target genes of DE\u2010miRs in ECRSwNP vs control and non\u2010ECRSwNP vs control groups. The miRNA\u2010mRNA regulatory networks constructed with Cytoscape highlighted the roles of miR\u2010154, miR\u2010221, and miR\u2010223 family miRNAs relating to both ECRSwNP and non\u2010ECRSwNP, and the roles of the let\u20107 and miR\u201034\/449 families in the development of non\u2010ECRSwNP. Assessment using real\u2010time PCR for the expression of miRNAs and target genes demonstrated highly consistent data with the RNA sequencing data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ECRSwNP and non\u2010ECRSwNP patients express distinct miRNA\u2010mRNA regulatory networks compared with control subjects, thus providing potential targets for future development of novel therapeutic approaches for the management of CRSwNP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.22781","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T14:56:04Z","timestamp":1613919364000},"page":"1207-1219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Integrated miRNA and mRNA expression profiling reveals dysregulated miRNA\u2010mRNA regulatory networks in eosinophilic and non\u2010eosinophilic chronic rhinosinusitis with nasal 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