{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:23:07Z","timestamp":1781014987852,"version":"3.54.1"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T00:00:00Z","timestamp":1672876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of microbes in chronic rhinosinusitis (CRS) is poorly understood. We hypothesize that analyzing prior microbial exposures via assessing microbial protein serological reactivity in CRS versus controls may offer insights for CRS etiopathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We profiled IgG and IgA antibodies to individual microbial proteins in serum samples of CRS patients and controls using a novel high\u2010throughput microarray protein technology, Nucleic Acid Programmable Protein Array (NAPPA). The study was conducted on 118 subjects (39 CRS, 79 controls). A CRS\u2010focused NAPPA array, with 1557 potentially sero\u2010reactive microbial proteins elected from a pre\u2010screening of 6500 genes of interest was constructed. It included membrane\u2010associated proteins from 47 bacterial species and all proteins from 43 viral strains. Differences between CRS and controls were compared across individual antimicrobial antibodies and the species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Chronic rhinosinusitis patients had significantly elevated antimicrobial antibodies compared with controls. One bacterium (<jats:italic>Staphylococcus aureus<\/jats:italic>) and three viral strains (human metapneumovirus, human herpesvirus 5, and human herpesvirus 4) were identified as sources of the proteins that showed significantly elevated sero\u2010reactivity in CRS patients. Within CRS, patients with polyps had elevated antibodies against <jats:italic>S. aureus<\/jats:italic>, influenza A virus (H1N1, H3N2), and rhinovirus B14. CRS patients without polyps showed more antibodies against human herpesvirus 1 and vaccinia virus WR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Compared with healthy controls, CRS patients\u2019 serum samples showed significantly increased sero\u2010reactivity to both bacterial and viral proteins, reflecting recent or current infection or active colonization. Significantly higher antibodies against <jats:italic>S. aureus<\/jats:italic>, human metapneumovirus, human herpesvirus 5, and human herpesvirus 4 in CRS need further study.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.23107","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T04:09:30Z","timestamp":1670818170000},"page":"1503-1510","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Antibody responses to the host microbiome in chronic rhinosinusitis"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7593-2385","authenticated-orcid":false,"given":"Devyani","family":"Lal","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Mayo Clinic in Arizona  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lusheng","family":"Song","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tripti","family":"Brar","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Mayo Clinic in Arizona  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily K.","family":"Cope","sequence":"additional","affiliation":[{"name":"Pathogen and Microbiome Institute Northern Arizona University  Flagstaff Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Keim","sequence":"additional","affiliation":[{"name":"Pathogen and Microbiome Institute Northern Arizona University  Flagstaff Arizona USA"},{"name":"Pathogen and Microbiome Division Translational Genomics Research Institute  Flagstaff Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy","family":"Williams","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunro","family":"Chung","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"},{"name":"College of Health Solutions Arizona State University  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vel","family":"Murugan","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"LaBaer","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Mitchell","family":"Magee","sequence":"additional","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin20.601"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22741"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1210"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1097639"},{"issue":"1","key":"e_1_2_8_6_1","first-page":"S277","article-title":"Identification of antibody targets for tuberculosis serology using high\u2010density nucleic acid programmable protein arrays","volume":"16","author":"Song L","journal-title":"Mol Cell Proteomics"},{"key":"e_1_2_8_7_1","article-title":"Multi\u2010platform approach for microbial biomarker identification using borrelia burgdorferi as a model","volume":"9","author":"Pflughoeft KJ","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2640-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00060-16"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12666"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02806-12"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000358880"},{"issue":"4","key":"e_1_2_8_13_1","article-title":"Impact of respiratory virus infections in exacerbation of acute and chronic rhinosinusitis","volume":"17","author":"Sen TanK","year":"2017","journal-title":"Curr Allergy Asthma Rep"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21934"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02838.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.11.2340"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.301.6764.1299"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky006"}],"container-title":["International Forum of Allergy &amp; Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.23107","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/alr.23107","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alr.23107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T15:57:54Z","timestamp":1692287874000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/alr.23107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,5]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/alr.23107"],"URL":"https:\/\/doi.org\/10.1002\/alr.23107","archive":["Portico"],"relation":{},"ISSN":["2042-6976","2042-6984"],"issn-type":[{"value":"2042-6976","type":"print"},{"value":"2042-6984","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,5]]},"assertion":[{"value":"2022-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}