{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:11:26Z","timestamp":1788347486612,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several biological processes are regulated by miR\u2010200a\u20103p, including cell proliferation, migration, and epithelial\u2013mesenchymal transition (EMT). In this study we aimed to uncover the diagnostic value and molecular mechanisms of miR\u2010200a\u20103p in chronic rhinosinusitis with nasal polyps (CRSwNP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expressions of miR\u2010200a\u20103p were detected by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), Zinc finger E\u2010box binding homeobox 1 (ZEB1) levels were examined by qRT\u2010PCR and immunofluorescence staining. The interaction between miR\u2010200a\u20103p and ZEB1 was predicted by TargetScan Human 8.0 and confirmed by dual\u2010luciferase reporter assays. In addition, the effect of miR\u2010200a\u20103p and ZEB1 on EMT\u2010related makers and inflammation cytokines was assessed by qRT\u2010PCR and Western blotting in human nasal epithelial cells (hNEpCs) and primary human nasal mucosal epithelial cells (hNECs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that miR\u2010200a\u20103p was downregulated in non\u2010eosinophilic and eosinophilic CRSwNP patients when compared with controls. The diagnostic value of miR\u2010200a\u20103p in serum is reflected by the receiver operating characteristic curve and the 22\u2010item Sino\u2010Nasal Outcome Test. Bioinformatic analysis and luciferase reporter assay identified ZEB1 as a target of miR\u2010200a\u20103p. ZEB1 was more highly expressed in CRSwNP than in controls. Furthermore, miR\u2010200a\u20103p inhibitor or ZEB1 overexpression significantly suppressed the epithelial marker E\u2010cadherin; promoted the activation of vimentin, \u03b1\u2010spinal muscle atrophy, and N\u2010cadherin; and aggravated inflammation in hNEpCs. Knockdown of ZEB1 significantly alleviated the cellular remodeling caused by miR\u2010200a\u20103p inhibitor via the extracellular signal\u2010regulated kinase (ERK)\/p38 pathway in hNECs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miR\u2010200a\u20103p suppresses EMT and inflammation by regulating the expression of ZEB1 via the ERK\/p38 pathway. Our study presents new ideas for protecting nasal epithelial cells from tissue remodeling and finding a possible target for disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.23215","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T10:38:44Z","timestamp":1686825524000},"page":"41-56","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["miR\u2010200a\u20103p regulates epithelial\u2013mesenchymal transition and inflammation in chronic rhinosinusitis with nasal polyps by targeting ZEB1 via ERK\/p38 pathway"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9751-2461","authenticated-orcid":false,"given":"Yisha","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2013Head and Neck Surgery Shandong Provincial ENT Hospital Shandong University Jinan Shandong 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