{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:31:07Z","timestamp":1785760267615,"version":"3.56.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:00:00Z","timestamp":1697241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["ZEMKE19A0"],"award-info":[{"award-number":["ZEMKE19A0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["BOMBER19R0"],"award-info":[{"award-number":["BOMBER19R0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic rhinosinusitis (CRS) is common in individuals with cystic fibrosis (CF) and is marked by chronic inflammation and episodes of infection that negatively impact quality of life. Several studies have shown that elexacaftor\u2013tezacaftor\u2013ivacaftor (ETI) improves symptoms and examination findings in CF\u2010CRS. The current study determines the effect of ETI on the sinonasal microbiota in CF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sinonasal samples were collected under endoscopic visualization before and after starting ETI. Samples were subjected to 16S amplicon sequencing and sequences were processed with the QIIME2 pipeline with subsequent analysis using the vegan R\u2010package.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010nine individual baseline samples and 23 sample pairs pre\u2010\/post\u2010ETI were available. At baseline, the cohort had samples dominated by <jats:italic>Staphylococcus<\/jats:italic>, and alpha diversity was lower than that of a published reference set of individuals without sinonasal disease. Individuals with prior sinus surgery had lower alpha diversity as measured by Shannon Index, Observed Richness, and Faith's phylogenetic diversity Index. Beta diversity differed between individuals with and without allergic rhinitis, with higher <jats:italic>Staphylococcus<\/jats:italic> abundance in those with allergic rhinitis. No change in alpha or beta diversity was seen after a median of 9 months on ETI. With ETI, the <jats:italic>Pseudomonas<\/jats:italic> genus and the genus containing <jats:italic>Burkholderia<\/jats:italic> decreased in samples containing these taxa at baseline. <jats:italic>Pseudomonas<\/jats:italic> abundance decreased with treatment as measured by qPCR. Core sinonasal microbiome members <jats:italic>Staphylococcus<\/jats:italic>, <jats:italic>Corynebacterium<\/jats:italic>, and <jats:italic>Streptococcus<\/jats:italic> were unchanged, while <jats:italic>Moraxella<\/jats:italic> increased with ETI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Treatment with ETI leads to a reduction in <jats:italic>Pseudomonas<\/jats:italic> abundance within the sinonasal microbiome of individuals with <jats:italic>Pseudomonas<\/jats:italic> at baseline.<\/jats:p><\/jats:sec>","DOI":"10.1002\/alr.23288","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T15:54:06Z","timestamp":1697298846000},"page":"928-938","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Elexacaftor\u2013tezacaftor\u2013ivacaftor decreases pseudomonas abundance in the sinonasal microbiome in cystic fibrosis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7722-6708","authenticated-orcid":false,"given":"Anna C.","family":"Zemke","sequence":"first","affiliation":[{"name":"Division of Pulmonary, Allergy, Critical Care and Sleep Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Hilliam","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; 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Neck Surgery University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M.","family":"Pilewski","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, Critical Care and Sleep Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1503-3150","authenticated-orcid":false,"given":"Brent A.","family":"Senior","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Division of Otolaryngology\u2013Head &amp; Neck Surgery Department of Surgery Harvard Medical School, Brigham and Women's Hospital  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaughn S.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2016.03.004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2013.10.011"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12924"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCF.2022.03.002"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1513\/ANNALSATS.202101\u2010057OC"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2020.07.002"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ALR.22716"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCF.2022.03.011"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ALR.22043"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40309"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22332"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/LARY.29409"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201053975\u20109"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ALL.14276"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/SPECTRUM.01251\u201022"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2017.08.007"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010017\u20130266\u20136"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/alr.21871"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MSPHERE.00679\u201018"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2021.03.022"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.104711"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20134486.2009.01995.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.8"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41587\u2010019\u20130209\u20139"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKS1219"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/wics.147"},{"key":"e_1_2_5_28_1","unstructured":"OksanenJ SimpsonG BlanchetF et\u00a0al.vegan: community ecology package. 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