{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:17:39Z","timestamp":1791339459784,"version":"4.3.1"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970858"],"award-info":[{"award-number":["81970858"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160209"],"award-info":[{"award-number":["82160209"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2024NSFSC1514"],"award-info":[{"award-number":["2024NSFSC1514"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mast cells (MCs) are involved in type 2 inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP), which depends on interleukin (IL)\u201033 stimulation. MiR\u2010221 is reported to be an important regulator of MCs, and miR\u2010221\u20103p can be expressed in CRSwNP. However, the role of miR\u2010221\u20103p in CRSwNP is unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Ethmoid tissues from control subjects (\n                      <jats:italic>n<\/jats:italic>\n                      = 12) and polyps from patients with CRSwNP (\n                      <jats:italic>n<\/jats:italic>\n                      = 40) were collected. The expression of miR\u2010221\u20103p and cytokines was detected by real\u2010time quantitative polymerase chain reaction (qPCR). The activation of P65 and ERK was determined by western blotting. The localization of miR\u2010221\u20103p was detected via in situ hybridization combined with immunofluorescence (IF), and its target was identified via a luciferase reporter system. Human MCs were incubated with IL\u201033 or stem cell factor. MicroRNA mimics\/inhibitor and lentiviral plasmids were used to determine the role of miR\u2010221\u20103p in MCs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We observed increased expression of miR\u2010221\u20103p in CRSwNP, and localized its expression in MCs. The expression of miR\u2010221\u20103p was negatively correlated with that of IL\u20104, IL\u20105, and IL\u201013 in CRSwNP. MiR\u2010221\u20103p can be induced by IL\u201033 in MCs and plays a negative regulatory role in cytokine expression and signaling pathways in IL\u201033\u2010induced MC activation. As the direct target of miR\u2010221\u20103p, the receptor KIT was negatively correlated with miR\u2010221\u20103p and decreased in CRSwNP. In MCs, KIT is essential for an effective response to IL\u201033 stimulation. We here demonstrated that miR\u2010221\u20103p regulates cytokine expression by targeting KIT in IL\u201033\u2010activated MCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MiR\u2010221\u20103p inhibits MC\u2010dependent type 2 inflammatory conditions, rendering it a negative regulator of CRSwNP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.23558","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T16:46:24Z","timestamp":1743093984000},"page":"837-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MiR\u2010221\u20103p Attenuates IL\u201033\u2010Induced Mast Cell Cytokine Expression by Targeting KIT"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2379-1549","authenticated-orcid":false,"given":"Ruowu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery West China Hospital, Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Medicine and Engineering Interdisciplinary Research Laboratory of Nursing &amp; Materials West China Hospital, Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2565-9103","authenticated-orcid":false,"given":"Jing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery West China Hospital, Sichuan University Chengdu 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