{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T14:44:04Z","timestamp":1769697844121,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Periostin is an extracellular matrix protein that is highly expressed in nasal polyp (NP) tissues in patients with chronic rhinosinusitis (CRS). This study investigated the cellular source of periostin in sinonasal tissues and the mechanism of IL\u201013\u2010induced periostin production in nasal epithelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The amount and localization of the periostin protein and mRNA were examined via immunohistochemistry and in situ hybridization analysis in control subjects and subjects with eosinophilic chronic rhinosinusitis (ECRS) and non\u2010ECRS. The expression levels of periostin and type 2 inflammatory cytokines in the tissue were measured by protein and mRNA levels. Normal human nasal epithelial cells were cultured in the presence of IL\u201013, and the effect of the specific inhibitors, leflunomide, PD98059, or PS1145, on periostin production was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Periostin was mainly deposited in the subepithelial region of NP tissues in ECRS and was more highly expressed than in non\u2010ECRS (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.01) and control subjects (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). The number of periostin mRNA\u2010positive basal epithelial cells was significantly increased in NP tissues in ECRS. The IL\u201013 concentration was strongly correlated with periostin expression in the tissues (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001,\n                      <jats:italic>r<\/jats:italic>\n                      = 0.8802). Periostin production was much greater in differentiated cells under long\u2010term stimulation with IL\u201013 than in ciliated cells, and this effect was attenuated by leflunomide and PD98059, but not by PS1145.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NP tissues from ECRS patients exhibit increased type 2 inflammatory cytokines and periostin. Nasal epithelial cells, particularly basal cells, are an important source of periostin. Exposure to IL\u201013 is important for periostin production from nasal epithelial cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.23559","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T05:37:53Z","timestamp":1741757873000},"page":"767-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["IL\u201013\u2010Induced Periostin Expression is Increased in Eosinophilic Chronic Rhinosinusitis"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9078-1001","authenticated-orcid":false,"given":"Isao","family":"Suzaki","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery School of Medicine Showa University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiko","family":"Hirabayashi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology 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