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While neurotrophins, particularly brain\u2010derived neurotrophic factor (BDNF), have been implicated in eosinophilic inflammation in allergic conditions, their involvement in CRSwNP remains undefined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bulk\u2010RNA sequencing of 23 nasal mucosa samples and ELISA quantification of 95 nasal secretions were conducted to evaluate BDNF expression across CRSwNP endotypes and its correlation with eosinophil infiltration and clinical severity. Cellular BDNF sources were mapped using immunofluorescence staining and single\u2010cell RNA sequencing. Primary human nasal epithelial cells (HNECs) were stimulated to investigate cytokine\u2010mediated BDNF regulation, while recombinant BDNF was applied to assess its functional effects on eosinophil survival, activation, and migration via flow cytometry and transwell assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Transcriptomic profiling linked BDNF to Th2\u2010skewed and eosinophilic inflammation signatures. Tissue and secretory BDNF levels were elevated in eosinophilic CRSwNP (ECRSwNP) versus controls, showing strong correlations with eosinophil infiltration and disease severity. BDNF level in nasal secretion demonstrated moderate diagnostic accuracy for ECRSwNP. IL\u20104\/IL\u201013 stimulation upregulated BDNF via JAK\/STAT signaling in HNECs. Exogenous BDNF enhanced eosinophil survival and migration through its high\u2010affinity receptor, tropomyosin receptor kinase B (TrkB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study positions BDNF as a key orchestrator of epithelial\u2012eosinophil crosstalk in CRSwNP, perpetuating inflammation through Th2\u2010primed BDNF secretion and eosinophil persistence. Therapeutic modulation of this axis may offer novel precision strategies for eosinophilic CRSwNP management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.23628","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T10:48:28Z","timestamp":1751453308000},"page":"1217-1227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["BDNF as a Key Mediator in Eosinophilic Inflammation in CRSwNP: Insights From Transcriptomic and Functional Analysis"],"prefix":"10.1002","volume":"15","author":[{"given":"Qinqin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery and Department of Allergy Beijing TongRen Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Institute of Otolaryngology Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission, Beijing Key Laboratory of New Medicine and Diagnostic Technology Research for Nasal Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery and Department of Allergy Beijing TongRen Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Institute of Otolaryngology Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission, Beijing Key Laboratory of New Medicine and Diagnostic Technology Research for Nasal Disease  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sicen","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery and Department of Allergy Beijing TongRen Hospital, Capital Medical University  Beijing China"},{"name":"Beijing Institute of Otolaryngology Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission, Beijing Key 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