{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:25:45Z","timestamp":1786976745964,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301288"],"award-info":[{"award-number":["82301288"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7252107"],"award-info":[{"award-number":["7252107"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M730330"],"award-info":[{"award-number":["2023M730330"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Forum Allergy Rhinol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Elevated immunoglobulin (Ig) E has been frequently observed in eosinophilic chronic rhinosinusitis with nasal polyps (eCRSwNP). However, the relationship between IgE levels and immune microenvironment remains poorly understood. This study aimed to characterize the transcriptomic and immunologic profiles associated with different IgE levels in eCRSwNP, and to explore their potential clinical and biological significance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We enrolled 30 patients with eCRSwNP and stratified them into IgE\u2010high (tissue total IgE \u2265 34.55 kU\/L and serum total IgE \u2265 100 kU\/L,\n                      <jats:italic>n<\/jats:italic>\n                      = 18) and IgE\u2010low (below both thresholds,\n                      <jats:italic>n<\/jats:italic>\n                      = 12) groups. Healthy nasal mucosa from 10 control subjects was included. Bulk RNA sequencing, gene ontology (GO), KEGG pathway analysis, and immunohistochemistry were performed to characterize IgE\u2010associated transcriptional and protein expression profiles in nasal polyp tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared with controls, IgE\u2010high patients exhibited higher tissue\u2010specific IgE levels against aeroallergens, more severe clinical manifestations, and greater asthma comorbidity. Transcriptomic profiling revealed distinct gene expression patterns between eCRSwNP and healthy mucosa, with enrichment of T2 inflammation markers (CCL13, POSTN, IL5, IGHG4, and IGHE). IgE\u2010high polyps demonstrated mucus hypersecretion and increased antimicrobial peptide expression (ITLN1, LTF, LYZ), alongside downregulated ciliary function\u2013related genes. Immunohistochemistry validated increased IgE, IgG4, CD79a\u207a B cells and reduced FOXJ1\u207a ciliated cells in IgE\u2010high tissues, consistent with the transcriptomic findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Tissue IgE levels delineate clinically and transcriptionally distinct eCRSwNP endotypes. IgE\u2010high nasal polyps demonstrated epithelial barrier dysfunction, mucus overproduction, and antimicrobial peptide activation, highlighting the potential of IgE\u2010based stratification to guide personalized treatment strategies in eCRSwNP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alr.70048","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:59:35Z","timestamp":1762282775000},"page":"170-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptomic Profiling Reveals Differences in Mucosal Defense and Ciliary Gene Expression in Eosinophilic Nasal Polyps Stratified by IgE Levels"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1724-2412","authenticated-orcid":false,"given":"Jinbo","family":"Han","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-1769","authenticated-orcid":false,"given":"Yuzhuo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9440-1117","authenticated-orcid":false,"given":"Weiqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8686-8764","authenticated-orcid":false,"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Peking Union Medical College  Beijing 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