{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:33:46Z","timestamp":1790354026805,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013301","name":"Parkinson's Foundation","doi-asserted-by":"publisher","award":["PF\u2010PRF\u2010934916"],"award-info":[{"award-number":["PF\u2010PRF\u2010934916"]}],"id":[{"id":"10.13039\/100013301","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>For routine clinical implementation of Alzheimer's disease (AD) plasma biomarkers, fully automated random\u2010access platforms are crucial to ensure reproducible measurements. We aimed to perform an\u00a0analytical validation and to establish\u00a0cutoffs for AD plasma biomarkers measured with Lumipulse.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      Two cohorts were included.\u00a0UNIPG:\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0450 paired cerebrospinal fluid (CSF)\/plasma samples from subjects along the AD\u2010continuum, subjects affected by other neurodegenerative diseases, and controls with known CSF profile;\u00a0AMS:\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a040 plasma samples from AD and\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a040 controls. Plasma\u00a0amyloid \u03b2 (A\u03b2)42, A\u03b240, and p\u2010tau181 were measured with Lumipulse. We evaluated analytical and diagnostic performance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Lumipulse assays showed high analytical performance. Plasma p\u2010tau181 levels accurately reflected CSF A+\/T+ profile in AD\u2010dementia and mild cognitive impairment (MCI)\u2010AD, but not in asymptomatic\u2010AD. Plasma and CSF A\u03b242\/40 values were concordant across clinical AD stages. Cutoffs and probability\u2010based models performed satisfactorily in both cohorts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>The identified\u00a0cutoffs and probability\u2010based models represent a significant step toward plasma AD molecular\u00a0diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.13687","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T08:15:17Z","timestamp":1707293717000},"page":"2453-2468","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Fully automated measurement of plasma A\u03b242\/40 and p\u2010tau181: Analytical robustness and concordance with cerebrospinal fluid profile along the Alzheimer's disease continuum in two independent cohorts"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0456-5650","authenticated-orcid":false,"given":"Giovanni","family":"Bellomo","sequence":"first","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3480-5136","authenticated-orcid":false,"given":"Sherif","family":"Bayoumy","sequence":"additional","affiliation":[{"name":"Neurochemistry Laboratory Department of Laboratory Medicine Amsterdam Neuroscience, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3148-9568","authenticated-orcid":false,"given":"Alfredo","family":"Megaro","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9500-7632","authenticated-orcid":false,"given":"Andrea","family":"Toja","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9123-9944","authenticated-orcid":false,"given":"Giovanna","family":"Nardi","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3967-8954","authenticated-orcid":false,"given":"Lorenzo","family":"Gaetani","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7305-2122","authenticated-orcid":false,"given":"Elena R.","family":"Blujdea","sequence":"additional","affiliation":[{"name":"Neurochemistry Laboratory Department of Laboratory Medicine Amsterdam Neuroscience, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9623-1900","authenticated-orcid":false,"given":"Federico","family":"Paolini Paoletti","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6958-8223","authenticated-orcid":false,"given":"Anna Lidia","family":"Wojda\u019aa","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1169-3258","authenticated-orcid":false,"given":"Davide","family":"Chiasserini","sequence":"additional","affiliation":[{"name":"Section of Biochemistry Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8766-6224","authenticated-orcid":false,"given":"Wiesje M.","family":"van der Flier","sequence":"additional","affiliation":[{"name":"Alzheimer Center Department of Neurology Vrije Universiteit Amsterdam, Amsterdam UMC  Amsterdam The Netherlands"},{"name":"Department of Epidemiology and Data Science Vrije Universiteit Amsterdam Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0341-7445","authenticated-orcid":false,"given":"Inge M. W.","family":"Verberk","sequence":"additional","affiliation":[{"name":"Neurochemistry Laboratory Department of Laboratory Medicine Amsterdam Neuroscience, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4061-0837","authenticated-orcid":false,"given":"Charlotte","family":"Teunissen","sequence":"additional","affiliation":[{"name":"Neurochemistry Laboratory Department of Laboratory Medicine Amsterdam Neuroscience, Amsterdam UMC  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5722-3967","authenticated-orcid":false,"given":"Lucilla","family":"Parnetti","sequence":"additional","affiliation":[{"name":"Center for Memory Disturbances Lab of Clinical Neurochemistry Section of Neurology Department of Medicine and Surgery University of Perugia  Perugia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1202753"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(21)00361\u20106"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2018.02.018"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010020\u20100762\u20102"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26801"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010021\u201001382\u2010x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010021\u201000939\u20109"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac333"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12756"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.647783"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2023.0199"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13014"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010132306"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010170850"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/trc2.12132"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2015.00179"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1037\/1082\u2010989X.12.4.399"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201012\u201077"},{"key":"e_1_2_9_20_1","unstructured":"FengD ManevskiD PermeMP.auRoc: various methods to estimate the AUC. Published online April 4 2020. Accessed January 5 2024.https:\/\/cran.r-project.org\/web\/packages\/auRoc\/index.html"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/83.4.835"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm\u20102021\u20100651"},{"key":"e_1_2_9_23_1","unstructured":"Delignette\u2010MullerM\u2010L DutangC PouillotR DenisJB SiberchicotA.fitdistrplus: Help to fit of a parametric distribution to non\u2010censored or censored data2023. Published online April 25 2023. Accessed January 5 2024.https:\/\/cran.r\u2010project.org\/web\/packages\/fitdistrplus\/index.html"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10121015"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000207371"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41531\u2010023\u201000509\u2010w"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2023.2338"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12545"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2021.3180"}],"container-title":["Alzheimer's &amp; Dementia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.13687","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:52:26Z","timestamp":1759935146000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/alz.13687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,7]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/alz.13687"],"URL":"https:\/\/doi.org\/10.1002\/alz.13687","archive":["Portico"],"relation":{},"ISSN":["1552-5260","1552-5279"],"issn-type":[{"value":"1552-5260","type":"print"},{"value":"1552-5279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,7]]},"assertion":[{"value":"2023-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}