{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:54:07Z","timestamp":1791863647623,"version":"4.3.4"},"reference-count":75,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>INTRODUCTION<\/jats:title><jats:p>Blood protein biomarkers demonstrate potential for Alzheimer's disease (AD) diagnosis. Limited studies examine the molecular changes in AD blood cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Bulk RNA\u2010sequencing of blood cells was performed on AD patients of Chinese descent (<jats:italic>n<\/jats:italic>\u00a0=\u00a0214 and 26 in the discovery and validation cohorts, respectively) with normal controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a0208 and 38 in the discovery and validation cohorts, respectively). Weighted gene co\u2010expression network analysis (WGCNA) and deconvolution analysis identified AD\u2010associated gene modules and blood cell types. Regression and unsupervised clustering analysis identified AD\u2010associated genes, gene modules, cell types, and established AD classification models.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>WGCNA on differentially expressed genes revealed 15 gene modules, with 6 accurately classifying AD (areas under the receiver operating characteristics curve [auROCs]\u00a0&gt;\u00a00.90). These modules stratified AD patients into subgroups with distinct disease states. Cell\u2010type deconvolution analysis identified specific blood cell types potentially associated with AD pathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>This study highlights the potential of blood transcriptome for AD diagnosis, patient stratification, and mechanistic studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>We comprehensively analyze the blood transcriptomes of a well\u2010characterized Alzheimer's disease cohort to identify genes, gene modules, pathways, and specific blood cells associated with the disease.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Blood transcriptome analysis accurately classifies and stratifies patients with Alzheimer's disease, with some gene modules achieving classification accuracy comparable to that of the plasma ATN biomarkers.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Immune\u2010associated pathways and immune cells, such as neutrophils, have potential roles in the pathogenesis and progression of Alzheimer's disease.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/alz.13691","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T10:15:29Z","timestamp":1707300929000},"page":"2469-2484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Using blood transcriptome analysis for Alzheimer's disease diagnosis and patient stratification"],"prefix":"10.1002","volume":"20","author":[{"given":"Huan","family":"Zhong","sequence":"first","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Guangdong Provincial Key Laboratory of Brain Science Disease and Drug Development HKUST Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyebin","family":"Uhm","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Cao","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Guangdong Provincial Key Laboratory of Brain Science Disease and Drug Development HKUST Shenzhen Research Institute  Shenzhen Guangdong China"},{"name":"The Brain Cognition and Brain Disease Institute Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen\u2013Hong Kong Institute of Brain Science\u2010Shenzhen Fundamental Research Institutions  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany T. W.","family":"Mak","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronnie Ming Nok","family":"Lo","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie Wing Yan","family":"Wong","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine Yee Ling","family":"Cheng","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kin Y.","family":"Mok","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Department of Neurodegenerative Disease UCL Institute of Neurology  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew Lung Tat","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Medicine Queen Elizabeth Hospital HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy C. Y.","family":"Kwok","sequence":"additional","affiliation":[{"name":"Therese Pei Fong Chow Research Centre for Prevention of Dementia Division of Geriatrics Department of Medicine and Therapeutics The Chinese University of Hong Kong  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent C. T.","family":"Mok","sequence":"additional","affiliation":[{"name":"Lau Tat\u2010chuen Research Centre of Brain Degenerative Diseases in Chinese Therese Pei Fong Chow Research Centre for Prevention of Dementia Gerald Choa Neuroscience Institute Li Ka Shing Institute of Health Sciences Division of Neurology Department of Medicine and Therapeutics The Chinese University of Hong Kong  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny C. F.","family":"Ip","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Guangdong Provincial Key Laboratory of Brain Science Disease and Drug Development HKUST Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hardy","sequence":"additional","affiliation":[{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Department of Neurodegenerative Disease UCL Institute of Neurology  London UK"},{"name":"Institute for Advanced Study The Hong Kong University of Science and Technology  HKSAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy K. Y.","family":"Fu","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Guangdong Provincial Key Laboratory of Brain Science Disease and Drug Development HKUST Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2763-8907","authenticated-orcid":false,"given":"Nancy Y.","family":"Ip","sequence":"additional","affiliation":[{"name":"Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience Center The Hong Kong University of Science and Technology  HKSAR China"},{"name":"Hong Kong Center for Neurodegenerative Diseases InnoHK  HKSAR China"},{"name":"Guangdong Provincial Key Laboratory of Brain Science Disease and Drug Development HKUST Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12328"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010018\u20100079\u20107"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2018.10.025"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(21)00361\u20106"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ALZ.12756"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010019\u20100795\u20102"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JALZ.2019.06.4951"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12369"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914257107"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107908"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13195\u2010022\u201000975\u2010Z\/FIGURES\/3"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016266"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2017.08.012"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010016\u20100355\u20103"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010020\u201000392\u20106"},{"key":"e_1_2_12_17_1","doi-asserted-by":"crossref","unstructured":"ZhaoY XieYZ LiuYS Accelerated aging\u2010related transcriptome alterations in neurovascular unit cells in the brain of Alzheimer's disease.Front Aging Neurosci.2022;14:949074. doi:10.3389\/fnagi.2022.949074","DOI":"10.3389\/fnagi.2022.949074"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100457\u20108"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532\u20105415.2005.53221.x"},{"key":"e_1_2_12_20_1","first-page":"612","article-title":"Association, AP diagnostic and statistical manual of mental disorders","volume":"5","author":"Arlington VA","year":"2013","journal-title":"Am Psychiatr Assoc"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/apnr.2000.9231"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.005"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.008"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1910-1"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1038"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20109\u2010559"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-77"},{"key":"e_1_2_12_31_1","doi-asserted-by":"crossref","unstructured":"KassambaraA MundtF Package \u2018factoextra.\u2019Extract and visualize the results of multivariate data analyses.2017;76(2).","DOI":"10.32614\/CRAN.package.factoextra"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100114\u20102"},{"key":"e_1_2_12_33_1","unstructured":"MaechlerM RousseeuwP CrouxC et\u00a0al.Package \u2018robustbase.\u2019Basic Robust StatAvailable online:https:\/\/cran.r\u2010project.org\/web\/packages\/robustbase\/index.html(accessed on 10 October 2021). Published online 2022."},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201010945\u2010z"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43856\u2010023\u201000269\u2010x"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.176552.114"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715554115"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1086\/521987"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.117259.110"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12369"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giaa151"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015543\u2010y"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2200154"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/dad2.12074"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12369"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201022491\u20108"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.01.053"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2021.02.001"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010019\u20100358\u20102"},{"key":"e_1_2_12_52_1","doi-asserted-by":"crossref","unstructured":"ZichaS BatemanRJ ShawLM et\u00a0al.Comparative analytical performance of multiple plasma A\u03b242 and A\u03b240 assays and their ability to predict positron emission tomography amyloid positivity.Alzheimer\u2019 Dement.2023;19(3):956\u2010966. doi:10.1002\/alz.12697","DOI":"10.1002\/alz.12697"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp\u20102020\u2010325537"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101346"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X16666180926123722"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.06.011"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010020\u20100696\u20100"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010015\u20100750\u2010x"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2006.08.004"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010022\u201000975\u2010z"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010020\u201000654\u2010x"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010018\u20100284\u20102"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010021\u201000549\u2010x"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380\u2010022\u201001637\u20100"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1159\/000341583"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/NM.3913"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25159"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13195\u2010020\u201000654\u2010X\/FIGURES\/7"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JNEUROIM.2020.577398"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010022\u201001347\u20102"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)31403\u20109"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010023\u201001169\u2010x"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/LIFE12091440"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010021\u201000549\u2010x"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.1604032113\/\u2010\/DCSUPPLEMENTAL"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2020.00456\/BIBTEX"}],"container-title":["Alzheimer's &amp; Dementia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.13691","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:52:29Z","timestamp":1759935149000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/alz.13691"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,7]]},"references-count":75,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/alz.13691"],"URL":"https:\/\/doi.org\/10.1002\/alz.13691","archive":["Portico"],"relation":{},"ISSN":["1552-5260","1552-5279"],"issn-type":[{"value":"1552-5260","type":"print"},{"value":"1552-5279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,7]]},"assertion":[{"value":"2023-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}