{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:58:54Z","timestamp":1759971534441,"version":"build-2065373602"},"reference-count":5,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T00:00:00Z","timestamp":1707350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alzheimer's disease (AD) represents a growing global health challenge, necessitating accurate and reliable diagnostic methodologies for timely intervention and management. Immunoassays, specifically designed to detect biomarkers associated with AD pathology, have emerged as pivotal tools in diagnostic development. Understanding of the established protocols ensures assay sensitivity, specificity, and reproducibility, thereby enhancing the clinical utility of these diagnostic tools. Here, we explore the considerations in immunoassay development, focusing on phosphorylated tau217 assays.<\/jats:p><jats:p>Ultimately, a clear understanding of immunoassay development is paramount in advancing the precision and reliability of AD diagnostics, contributing to early detection, improved patient outcomes, and advancements in therapeutic interventions.<\/jats:p>","DOI":"10.1002\/alz.13708","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T05:19:03Z","timestamp":1707369543000},"page":"3114-3115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Analytical and clinical validation of Alzheimer's disease blood biomarkers with a focus on plasma p\u2010tau217"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7897-9456","authenticated-orcid":false,"given":"Fernando","family":"Gonzalez\u2010Ortiz","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology the Sahlgrenska Academy at the University of Gothenburg  M\u00f6lndal Sweden"},{"name":"Clinical Neurochemistry Laboratory Sahlgrenska University Hospital  M\u00f6lndal Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K.","family":"Karikari","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology the Sahlgrenska Academy at the University of Gothenburg  M\u00f6lndal Sweden"},{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaj","family":"Blennow","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology the Sahlgrenska Academy at the University of Gothenburg  M\u00f6lndal Sweden"},{"name":"Clinical Neurochemistry Laboratory Sahlgrenska University Hospital  M\u00f6lndal Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,8]]},"reference":[{"key":"e_1_2_1_4_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13525"},{"key":"e_1_2_1_4_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2015.00179"},{"issue":"7","key":"e_1_2_1_4_4_1","first-page":"757","article-title":"Linearity and calibration. a clinical laboratory perspective","volume":"116","author":"Passey RB","year":"1992","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_4_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac407"},{"key":"e_1_2_1_4_6_1","unstructured":"ALZFORUM.Plasma p\u2010Tau\u2010217 Assays Work Well But No Home Run for Diagnosis. Accessed on December 17 2023.https:\/\/www.alzforum.org\/news\/conference\u2010coverage\/plasma\u2010p\u2010tau\u2010217\u2010assays\u2010work\u2010well\u2010no\u2010home\u2010run\u2010diagnosis"}],"container-title":["Alzheimer's &amp; Dementia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.13708","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:55:22Z","timestamp":1759935322000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/alz.13708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,8]]},"references-count":5,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/alz.13708"],"URL":"https:\/\/doi.org\/10.1002\/alz.13708","archive":["Portico"],"relation":{},"ISSN":["1552-5260","1552-5279"],"issn-type":[{"type":"print","value":"1552-5260"},{"type":"electronic","value":"1552-5279"}],"subject":[],"published":{"date-parts":[[2024,2,8]]},"assertion":[{"value":"2023-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}