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Novel astroglial PET\u2010tracers BU99008 and Deprenyl, hold immense potential for visualizing reactive astrogliosis in PD.\u00a0 However, they have not been thoroughly investigated in PD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      We employed a multi\u2010marker approach and performed in vitro radioligand binding and autoradiography studies with\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010BU99008 and\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010Deprenyl together with astrocytic immunofluorescence and morphometric analyses in the frontal cortex, temporal cortex, caudate and putamen brain regions of PD (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a04) and control (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a07) cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS AND DISCUSSION<\/jats:title>\n                    <jats:p>\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010BU99008 and\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010Deprenyl showed distinct binding behavior and displayed a diverse array of binding sites (single or multiple) in PD and control brains. Importantly,\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010BU99008 and\n                      <jats:sup>3<\/jats:sup>\n                      H\u2010Deprenyl autoradiography studies captured pronounced reactive astrogliosis in PD brain regions, corroborated by marked changes in astrocytic markers, morphology, and cellular processes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Astroglial tracers BU99008 and Deprenyl displayed a range of binding sites with different levels of affinity and proportions (%) in healthy control (CN) and Parkinson's disease (PD) brains.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Astroglial tracers BU99008 and Deprenyl showed a highly specific (permanent) high\u2010affinity (HA) binding site in the nanomolar range, which might be consistent across different pathologies.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Astroglial tracers BU99008 and Deprenyl highlighted distinct tracer binding behavior, indicating that they might be targeting different subpopulations or specific states of astrocytes in CN and PD brains.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Astroglial tracers BU99008 and Deprenyl captured prominent reactive astrogliosis at the advanced\/end stages of PD, substantiated by a significant increase in intercellular adhesion molecule 1 (ICAM\u20101)\u2013positive reactive astrocytes and marked changes in astrocytic morphology and processes.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.14488","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T07:00:22Z","timestamp":1739343622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mapping reactive astrogliosis in Parkinson's brain with astroglial tracers BU99008 and Deprenyl: New insights from a multi\u2010marker postmortem study"],"prefix":"10.1002","volume":"21","author":[{"given":"Filipa M.","family":"Rocha","sequence":"first","affiliation":[{"name":"Division of Clinical Geriatrics, Center for Alzheimer Research, Department of Neurobiology, Care Sciences and Society Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avishek","family":"Roy","sequence":"additional","affiliation":[{"name":"Division of Clinical 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