{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:51:54Z","timestamp":1782978714378,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 AG072973"],"award-info":[{"award-number":["P30 AG072973"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>INTRODUCTION<\/jats:title><jats:p>Analyzing the proteomes of different brain cell types is fundamental for understanding the pathophysiology of Alzheimer's disease (AD). However, spatial analysis of these diverse and limited cell populations poses significant challenges.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>The GeoMx Digital Spatial Profiler (DSP) platform was used to analyze protein level in the prefrontal cortex of AD and non\u2010AD brains. The platform interrogated 76 proteins and used immunofluorescence to distinguish between three cell types.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Neprilysin (NEP), which promotes amyloid beta degradation, was significantly higher in AD neurons and microglia. Lysosome\u2010associated membrane protein 2A (LAMP2A)\u00a0level was higher in neurons of individuals with AD compared to a control group. In addition, markers of neuroinflammation, such as CD11c, CD11b, and CD163, were also elevated in AD neurons.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>Our findings indicate the DSP platform effectively facilitates cell\u2010specific snapshots of the AD brain proteome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>The expression of 76 proteins was studied in neurons, astrocytes, and microglia.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We identified 18 differentially expressed proteins in AD neurons.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neprilysin was upregulated in neurons and microglia.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/alz.70339","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T11:11:14Z","timestamp":1749726674000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cell\u2010specific protein expression in Alzheimer's disease prefrontal cortex"],"prefix":"10.1002","volume":"21","author":[{"given":"Maryam","family":"Gholampour","sequence":"first","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine University of Kansas Medical Center  Kansas City Kansas USA"},{"name":"Alzheimer's Disease Research Center University of Kansas  Kansas City USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malay K.","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell H.","family":"Swerdlow","sequence":"additional","affiliation":[{"name":"Alzheimer's Disease Research Center University of Kansas  Kansas City USA"},{"name":"Department of Neurology University of Kansas Medical Center  Kansas City Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Haeri","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; 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