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It is unclear if this is due to the use of the same antibody (the \u201cALZpath antibody\u201d). We established and evaluated a novel p\u2010tau217 assay that uses an alternative antibody and benchmarked the results against ALZpath\u2010p\u2010tau217.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      After development and analytical validation of the University of Pittsburgh (\u201cPitt\u2010p\u2010tau217\u201d) method, clinical verification was performed in three independent cohorts (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0363).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Pitt\u2010p\u2010tau217 demonstrated high between\u2010run stability, linearity, and specificity. Clinically, Pitt\u2010p\u2010tau217 differentiated neuropathologically confirmed\n                      <jats:italic>PSEN1<\/jats:italic>\n                      mutation carriers from controls with area under the curve (AUC)\u00a0=\u00a00.94, and amyloid beta (A\u03b2) positron emission tomography (PET)\u2013positive from A\u03b2 PET\u2013negative cognitively normal older adults with AUC up to 0.84, equivalent to ALZpath\u2010p\u2010tau217 results. Both Pitt\u2010p\u2010tau217 and ALZpath\u2010p\u2010tau217 were slightly elevated in tau PET\u2013positive versus tau PET\u2013negative participants. Between\u2010assay correlations were up to 0.93.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>The new Pitt\u2010p\u2010tau217 assay exhibits high and reproducible classification accuracies for identifying individuals with biological evidence of Alzheimer's disease, equivalent to the widely used ALZpath\u2010p\u2010tau217.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>We designed and developed an alternative assay to quantify plasma phosphorylated tau (p\u2010tau)217, aiming to enhance accuracy and enable early detection of Alzheimer's disease (AD).<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Comprehensive analytical and clinical validation demonstrated that the new p\u2010tau217 assay is a valuable and affordable resource for investigating AD pathophysiology.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>The new p\u2010tau217 assay showed similar performance to the established ALZpath assay in staging and monitoring early AD.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.70409","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T03:41:50Z","timestamp":1752637310000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pittsburgh plasma p\u2010tau217: Classification accuracies for autosomal dominant and sporadic Alzheimer's disease in the community"],"prefix":"10.1002","volume":"21","author":[{"given":"Anuradha","family":"Sehrawat","sequence":"first","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric E.","family":"Abrahamson","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Geriatric Research Education and Clinical Center, VA Pittsburgh HS  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca A.","family":"Deek","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Health Data Science, School of Public Health University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gogola","sequence":"additional","affiliation":[{"name":"Department of Radiology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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