{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:00:58Z","timestamp":1791594058516,"version":"4.3.4"},"reference-count":91,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS139948"],"award-info":[{"award-number":["NS139948"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>\n                      Investigations of retinal biomarkers for Alzheimer's disease (AD) and AD and related dementias (ADRD), has increased significantly. We examine retinal vascular health in a mouse containing the ADRD risk variant\n                      <jats:italic>\n                        Mthfr\n                        <jats:sup>677C&gt;T<\/jats:sup>\n                      <\/jats:italic>\n                      to determine if changes in retina mirror similar changes in cerebrovasculature.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      Morphology and function of retinal vasculature and neurons were assessed using in vivo imaging, immunohistochemistry, and pattern electroretinography. RNAscope and proteomics were employed to determine\n                      <jats:italic>Mthfr<\/jats:italic>\n                      gene expression and differential protein expression in mice carrying\n                      <jats:italic>\n                        Mthfr\n                        <jats:sup>677C&gt;T<\/jats:sup>\n                      <\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Mice show age\u2010 and sex\u2010dependent retinal vascular deficits, displaying similarities to previously published brain data.\n                      <jats:italic>Mthfr<\/jats:italic>\n                      is widely expressed and co\u2010localizes with vascular cell markers. Proteomics identified common molecular signatures across the brain and retina.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>\n                      Results demonstrate that\n                      <jats:italic>Mthfr<\/jats:italic>\n                      \u2010dependent vascular phenotypes occur in brain and retina similarly. These data suggest that assessing age and genetic\u2010driven changes within retinal vasculature represents a minimally invasive method to predict AD\u2010related cerebrovascular damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>\n                              Mthfr\n                              <jats:sup>677C&gt;T<\/jats:sup>\n                            <\/jats:italic>\n                            retinal vascular phenotypes align with cerebrovascular phenotypes.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>\n                              Mthfr\n                              <jats:sup>677C&gt;T<\/jats:sup>\n                            <\/jats:italic>\n                            brain and retina share Alzheimer's disease (AD)\u2013)relevant differentially expressed proteins.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Retinal imaging may provide insight regarding genetic risk for vascular dysfunction.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.70501","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T10:20:01Z","timestamp":1753957201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Retinal vascular dysfunction in the\n                    <i>\n      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