{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:05:56Z","timestamp":1792641956587,"version":"4.5.0"},"reference-count":75,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD)\u2019s multifactorial nature stresses the role of epigenetics in affecting different pathological pathways. We demonstrated that one\u2010carbon metabolism epigenetically impacts AD\u2010like phenotype. Here, we investigated the crosstalk between methylation and microRNAs in AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      We altered one\u2010carbon metabolism to induce hypo\u2010 and hyper\u2010methylation, in SK\u2010N\u2010BE neuroblastoma cells and TgCRND8 mice. miRNAs were profiled through a polymerase chain reaction array, then we focused on\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      expression and methylation of its genomic locus. Finally, we assessed\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      expression and methylation in the brain of AD subjects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      <jats:italic>MiR\u201029a<\/jats:italic>\n                      was repressed in hypomethylating and expressed in hypermethylating conditions. The expression of\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      and of its target,\n                      <jats:italic>BACE1<\/jats:italic>\n                      , was inversely correlated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>\n                      We demonstrated for the first time that\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      is modulated by one\u2010carbon metabolism through DNA methylation, disclosing the molecular mechanisms regulating\n                      <jats:italic>BACE1<\/jats:italic>\n                      expression in AD. These data confirm\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      \u2019s protective role in AD and support\n                      <jats:italic>miR\u201029a<\/jats:italic>\n                      as a potential biomarker for AD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.70703","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T02:59:53Z","timestamp":1758682793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["One\u2010carbon metabolism modulates\n                    <i>miR\u201029a<\/i>\n                    \u2013DNA methylation crosstalk in Alzheimer's disease"],"prefix":"10.1002","volume":"21","author":[{"given":"Tiziana","family":"Raia","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria A.","family":"Cavallaro","sequence":"additional","affiliation":[{"name":"Department of Surgery Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Diniz Ferreira","family":"Borges","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Cinti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples \u201cFederico II\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Bizzarri","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isidre","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Experimental Therapeutics Emeritus Professor University of Barcelona Hospitalet de Llobregat  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Lucarelli","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome  Rome Italy"},{"name":"Pasteur Institute Cenci Bolognetti Foundation Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9467-5671","authenticated-orcid":false,"given":"Andrea","family":"Fuso","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome  Rome Italy"},{"name":"CRiN Center for Research in Neurobiology Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2024.149262"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13144100"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-240488"},{"issue":"24","key":"e_1_2_10_5_1","first-page":"00407","article-title":"The amyloid cascade hypothesis: a conclusion in search of support","volume":"0002","author":"Castellani RJ","year":"2024","journal-title":"Am J Pathol"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252212335"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2024.09.051"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13101208"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2024.1491952"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40035-024-00432-x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering11010045"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25115970"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2024.1398026"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2023-0416"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13668-019-0266-1"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"7","article-title":"The methionine\u2010homocysteine cycle and its effects on cognitive diseases","volume":"8","author":"Miller AL","year":"2003","journal-title":"Altern Med Rev"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002698"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2006-9406"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2011.01.012"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199448020-00002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2011.12.013"},{"issue":"1","key":"e_1_2_10_22_1","first-page":"39","article-title":"Folate, vitamin B12 and vitamin B6 and one carbon metabolism","volume":"6","author":"Selhub J","year":"2002","journal-title":"J Nutr Health Aging"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592294.2020.1722917"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.09.007"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2009.12794"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2020.02.006"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24087235"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2024.05.005"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2024.106717"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-024-03075-8"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102483"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14243"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.4103\/NRR.NRR-D-23-01566"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25137229"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.150768"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.52175"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2024.1423340"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3728"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S442876"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710263105"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00141.2011"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605751"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22630"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2017562118"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707628104"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140714647"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100710200"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.07.001"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118318"},{"key":"e_1_2_10_50_1","article-title":"The regulatory interplay between miRNA and DNA methylation orchestrates vital ovarian functions and associated traits in PCOS","author":"Bhingardeve S","year":"2024","journal-title":"Gene"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/17501911.2024.2370229"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-024-09774-1"},{"issue":"1","key":"e_1_2_10_53_1","first-page":"39","article-title":"Folate, vitamin B12 and vitamin B6 and one carbon metabolism","volume":"6","author":"Selhub J","year":"2002","journal-title":"J Nutr Health Aging"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/71.2.614s"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2024.020501"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c03542"},{"issue":"4","key":"e_1_2_10_57_1","first-page":"485","article-title":"A state\u2010of\u2010the\u2010art review on miRNA in prevention and treatment of Alzheimer\u2019s disease","volume":"52","author":"Guo X","year":"2023","journal-title":"Zhejiang Da Xue Xue Bao Yi Xue Ban"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2024.149090"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2024.02.025"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2024.01.028"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-024-05110-0"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-024-01341-1"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-024-02935-7"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14010165"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2024.102131"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2024.05.006"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2010-1284"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2010-091666"},{"issue":"1","key":"e_1_2_10_69_1","first-page":"27","article-title":"DNA methylation profiles of selected pro\u2010inflammatory cytokines in Alzheimer disease","volume":"76","author":"Nicolia V","year":"2017","journal-title":"J Neuropathol Exp Neurol"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1685"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1136352"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-025-03617-3"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2024.221"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-24-0977"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2013.08.001"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msard.2022.104426"}],"container-title":["Alzheimer's &amp; Dementia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.70703","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/alz.70703","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.70703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T11:08:52Z","timestamp":1771153732000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/alz.70703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":75,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/alz.70703"],"URL":"https:\/\/doi.org\/10.1002\/alz.70703","archive":["Portico"],"relation":{},"ISSN":["1552-5260","1552-5279"],"issn-type":[{"value":"1552-5260","type":"print"},{"value":"1552-5279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2025-02-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70703"}}