{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T07:09:13Z","timestamp":1784012953354,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01NS132181"],"award-info":[{"award-number":["U01NS132181"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS128843"],"award-info":[{"award-number":["R01NS128843"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 AG066514"],"award-info":[{"award-number":["P30 AG066514"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 AG13846"],"award-info":[{"award-number":["P30 AG13846"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Cerebrovascular alterations are associated with the pathology of Alzheimer's disease (AD). Yet, the role of these alterations is not fully understood, partly due to a paucity of data from mesoscopic vasculature (24\u2013240\u00a0\u00b5m diameter).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>We used label\u2010free, serial\u2010sectioning optical coherence tomography to reconstruct mesoscopic cerebrovasculature of the dorsolateral prefrontal cortex (DLPFC) samples from AD and control subjects. We quantified three\u2010dimensional alterations to the vascular networks and measured the correlations between vascular alterations and deposits of amyloid \u03b2 protein (A\u03b2) and phosphorylated tau protein (p\u2010tau).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The AD group had significantly reduced volume fraction, vessel length density, and branch density. There were negative trends between a subset of the vascular metrics and the density of A\u03b2 and p\u2010tau.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>AD samples in DLPFC present with significant mesoscopic cerebrovasculature alterations. This insight helps close the knowledge gap between micro\u2010 and macroscopic cerebrovascular pathologies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Volumetric imaging of mesoscopic cerebrovasculature with serial\u2010sectioning optical coherence tomography (OCT).<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Vascular metrics: volume fraction, length density, branch density, and tortuosity.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Human ex vivo samples from dorsolateral prefrontal cortex (Alxzheimer's disease [AD] and controls).<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Compared to controls, AD had reduced vascularity.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Serial\u2010sectioning OCT provides novel insights into mesoscopic cerebrovasculature.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.70907","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T03:57:19Z","timestamp":1763438239000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mesoscopic vascular alterations in Alzheimer's disease"],"prefix":"10.1002","volume":"21","author":[{"given":"Mackenzie","family":"Hyman","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaibin","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayang","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Radiology Massachusetts General Hospital, A.A. Martinos Center for Biomedical Imaging  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Chollet","sequence":"additional","affiliation":[{"name":"Department of Radiology Massachusetts General Hospital, A.A. Martinos Center for Biomedical Imaging  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarui","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Novoseltseva","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman A.","family":"Abdelhakeem","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u03cblce","family":"K\u03cbreli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josh","family":"Bevan","sequence":"additional","affiliation":[{"name":"Research Computing Services Boston University  Brookline Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Boston University School of Public Health  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Hof","sequence":"additional","affiliation":[{"name":"Nash Family Department of Neuroscience Friedman Brain Institute, and Ronald M. Loeb Center for Alzheimer's Disease, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann C.","family":"McKee","sequence":"additional","affiliation":[{"name":"Veterans Affairs (VA) Boston Healthcare System US Department of Veteran Affairs  Boston Massachusetts USA"},{"name":"Alzheimer's Disease Research Center and Chronic Traumatic Encephalopathy Center Chobanian and Avedisian School of Medicine Boston University  Boston Massachusetts USA"},{"name":"Department of Neurology, Chobanian and Avedisian School of Medicine Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irving J.","family":"Bigio","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"},{"name":"Department of Electrical and Computer Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Boas","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"},{"name":"Department of Electrical and Computer Engineering Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand R.","family":"Huber","sequence":"additional","affiliation":[{"name":"Veterans Affairs (VA) Boston Healthcare System US Department of Veteran Affairs  Boston Massachusetts USA"},{"name":"Alzheimer's Disease Research Center and Chronic Traumatic Encephalopathy Center Chobanian and Avedisian School of Medicine Boston University  Boston Massachusetts USA"},{"name":"Department of Neurology, Chobanian and Avedisian School of Medicine Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiology Massachusetts General Hospital, A.A. 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