{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:47:24Z","timestamp":1790938044768,"version":"4.1.0"},"reference-count":10,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp; Dementia"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n INTRODUCTION<\/jats:title>\n Plasma phosphorylated tau at threonine 217 (p\u2010tau217) has emerged as one of the most promising blood\u2010based biomarkers for Alzheimer's disease (AD). While rare, heterophilic antibodies (HAb) are a persistent potential confounding factor in immunoassays.<\/jats:p>\n <\/jats:sec>\n \n METHODS<\/jats:title>\n Potential HAb interference in the Lumipulse G pTau217 assay in samples exhibiting concentrations\u00a0>\u00a010 pg\/mL were investigated. Samples were subjected to HAb blocking reagent (HBT), and in some cases, polyethylene glycol (PEG) precipitation and serial dilutions.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n In 14 of the 15 suspected HAb cases, HBT treatment greatly reduced measured p\u2010tau217 concentrations. and in one of three specimens selected for further investigation,\u00a0reduced A\u03b242 concentrations PEG precipitation in the three selected samples also reduced p\u2010tau217 concentrations, while serial dilution yielded mixed effects. Control specimens were unaffected by sample treatment or dilutions.<\/jats:p>\n <\/jats:sec>\n \n DISCUSSION<\/jats:title>\n HAb may result in falsely elevated p\u2010tau217 and p\u2010tau217\/A\u03b242 ratio. HAb interference should be considered in cases of unusually high p\u2010tau217 concentrations prior to clinical interpretation.<\/jats:p>\n <\/jats:sec>\n \n Highlights<\/jats:title>\n \n \n \n Potential heterophile antibody (HAb) interference in the Lumipulse p\u2010tau217 immunoassay was investigated.<\/jats:p>\n <\/jats:list-item>\n \n Investigation was performed using HAb blocking reagent, and in some cases also using PEG precipitation .<\/jats:p>\n <\/jats:list-item>\n \n Patients with p\u2010tau217 concentrations\u00a0>\u00a010 pg\/mL exhibited positive HAb interference in 14 of 15 cases.<\/jats:p>\n <\/jats:list-item>\n \n HAb interference can lead to falsely positive p\u2010tau217\/A\u03b242 ratios.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/alz.71045","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T09:46:52Z","timestamp":1767001612000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["False positive plasma p\u2010tau217 results associated with heterophilic antibody interference: A potential clinical pitfall"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4455-6217","authenticated-orcid":false,"given":"Alicia","family":"Algeciras\u2010Schimnich","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Paul","family":"Theobald","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Figdore","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Ashrafzadeh Kian","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua A.","family":"Bornhorst","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Mayo Clinic Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2013.05.011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2903-1_7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-1288"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/47.7.1332"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hmedic.2024.100042"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14140"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(25)00227-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70316"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70535"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030122"}],"container-title":["Alzheimer's 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Dementia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.71045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/alz.71045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/alz.71045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T03:37:44Z","timestamp":1780285064000},"score":1,"resource":{"primary":{"URL":"https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/10.1002\/alz.71045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":10,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/alz.71045"],"URL":"https:\/\/doi.org\/10.1002\/alz.71045","archive":["Portico"],"relation":{},"ISSN":["1552-5260","1552-5279"],"issn-type":[{"value":"1552-5260","type":"print"},{"value":"1552-5279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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