{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:41:17Z","timestamp":1790728877690,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Biofluid Biomarkers Best Practices Workgroup of the National Alzheimer's Coordinating Center\u2013Alzheimer's Disease Research Center (ADRC) Biomarker Core Steering Committee was convened to update pre\u2010analytical handling guidelines for biofluid biomarkers, focusing on cerebrospinal fluid (CSF) and blood. We reviewed current literature pertinent to best practices for biomarker studies and surveyed the ADRCs for biomarker analytes, platforms, and protocols used at each center. Across 37 ADRCs, 16 CSF and 28 plasma\/serum analytes were reported to be studied at multiple centers. The pre\u2010analytical handling steps and concerns related to each, as supported by empirical studies and expert opinion, were integrated to generate a revised guideline document. The guideline aimed to standardize steps in biospecimen and biomarker analyte collection, storage, and pre\u2010analytical handling across the ADRCs. The 2025 ADRC guidelines represent the current working knowledge on biomarker best practices, providing guidance and harmonized protocols, and promoting robust analysis and reporting of composite data.<\/jats:p>","DOI":"10.1002\/alz.71252","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:40:03Z","timestamp":1777891203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Pre\u2010analytical guidelines for blood and CSF Biomarkers 2025: Recommendations from the NACC ADRC Biofluid Biomarker Best Practices Workgroup"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5524-4251","authenticated-orcid":false,"given":"Timothy E.","family":"Van Meter","sequence":"first","affiliation":[{"name":"BRAINBox Solutions , Inc.  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward N.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Neurology &amp; Neurological Sciences Stanford University  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Elahi","sequence":"additional","affiliation":[{"name":"Department of Neurology Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9245-8278","authenticated-orcid":false,"given":"Robert A.","family":"Rissman","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neuroscience Alzheimer's Therapeutic Research Institute Keck School of Medicine of the University of Southern California  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Argentina Lario","family":"Lago","sequence":"additional","affiliation":[{"name":"Memory and Aging Center Department of Neurology Weill Institute for Neurosciences University of California, San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelley","family":"Faber","sequence":"additional","affiliation":[{"name":"NCRAD Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Gauthreaux","sequence":"additional","affiliation":[{"name":"National Alzheimer's Coordinating Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Osorio","sequence":"additional","affiliation":[{"name":"Nathan S. Kline Institute for Psychiatric Research  Orangeburg New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Child Psychiatry Center for the Advancement of Children's Mental Health Columbia University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Gray","sequence":"additional","affiliation":[{"name":"Oregon ADRC Oregon Health and Sciences University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Morris","sequence":"additional","affiliation":[{"name":"KU ADRC KU Clinical Research Center  Fairway Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Liu","sequence":"additional","affiliation":[{"name":"Duke University School of Medicine Department of Neurology Durham North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Stockwell","sequence":"additional","affiliation":[{"name":"National Alzheimer's Coordinating Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Kukull","sequence":"additional","affiliation":[{"name":"Wisconsin ADRC  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7679-6282","authenticated-orcid":false,"given":"Rachael","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Neurology University of California, Davis  Sacramento California USA"},{"name":"Alzheimer's Disease Research Center University of California, Davis  Sacramento California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4362-2593","authenticated-orcid":false,"given":"Thomas S.","family":"Wingo","sequence":"additional","affiliation":[{"name":"Department of Translational Neuroscience College of Human Medicine Michigan State University  Grand Rapids Michigan USA"},{"name":"Michigan Alzheimer's Disease Research Center University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas M.","family":"Kanaan","sequence":"additional","affiliation":[{"name":"NCRAD Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Russ","sequence":"additional","affiliation":[{"name":"National Alzheimer's Coordinating Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Wilcock","sequence":"additional","affiliation":[{"name":"Nathan S. 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