{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:00:32Z","timestamp":1785477632691,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp;amp; Dementia"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>Proline\u2010rich tyrosine kinase 2 (PYK2) and focal adhesion kinase (FAK) are non\u2010receptor tyrosine kinases implicated in Alzheimer's disease (AD), but their functional role in microglia remains understudied. Selective pharmacological tools are required for preclinical studies leading to translational therapeutic development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>We evaluated potent and selective inhibitors described in publications and patents, synthesized or procured representative compounds, and profiled them in kinase assays. In vitro physicochemical and pharmacokinetic (PK) properties were assessed, and functional effects were studied in microglial phagocytosis assays using HMC3 and BV2 microglia cellular models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Biochemical profiling confirmed potent and selective inhibition, consistent with reported data, though assay\u2010dependent differences in apparent selectivity were observed. Most compounds showed favorable physicochemical and PK properties. In HMC3 assay, the PYK2\u2010selective tool compounds showed that strong stimulation of phagocytosis and parallel cell counts declined at non\u2010toxic concentrations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>This curated set of well\u2010characterized inhibitors provides a validated toolkit to probe PYK2\/FAK biology in AD\u2010relevant models.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.71508","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T03:29:17Z","timestamp":1780025357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Curated set of tool compounds to probe PYK2 and FAK signaling in Alzheimer's disease"],"prefix":"10.1002","volume":"22","author":[{"given":"Pathum M.","family":"Weerawarna","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Clinical Pharmacology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia D.","family":"Jesudason","sequence":"additional","affiliation":[{"name":"LGenia  Fortville Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Lobb","sequence":"additional","affiliation":[{"name":"LGenia  Fortville Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Clinical Pharmacology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Clinical Pharmacology Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyou","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Clinical Pharmacology Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Stark Neurosciences Research Institute, IUSM  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy I","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Clinical Pharmacology Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Stark Neurosciences Research Institute, IUSM  Indianapolis Indiana USA"},{"name":"Indiana Biosciences Research Institute  Indianapolis Indiana 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