{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T05:12:25Z","timestamp":1788671545990,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["alz-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Alzheimer's &amp; Dementia"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>INTRODUCTION<\/jats:title>\n                    <jats:p>\n                      Astrocyte dysfunction is central to Alzheimer's disease (AD), yet expression patterns of astrocytic markers remain poorly defined. We measured Aquaporin\u20104 (AQP4) and glial fibrillary acidic protein (GFAP) in\n                      <jats:italic>post\u2010mortem<\/jats:italic>\n                      frontal cortex of AD patients and controls across BrainNet Europe (BNE) stages.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>We assessed marker expression across gray and white matter with immunohistochemistry and immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>In AD, gray\u2010matter AQP4 area\u2013fraction did not differ significantly overall by immunohistochemistry, while a stage\u2010dependent increase emerged by BNE VI in both gray and white matter. AQP4\/amyloid\u2010\u03b2 (A\u03b2) and AQP4\/tau ratios were significantly reduced, consistent with reduced AQP4 retention relative to local proteinopathy burden. GFAP intensity was significantly decreased in both gray and white matter of AD patients, with disorganized peri\u2010plaque morphology in gray matter.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>DISCUSSION<\/jats:title>\n                    <jats:p>These findings reveal compartment\u2010 and stage\u2010specific astrocytic dysregulation in AD frontal cortex and identify local loss of AQP4 around proteinopathy. They support investigation of astrocyte\/glymphatic\u2010related pathways as biomarkers and therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/alz.71745","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:22:52Z","timestamp":1786699372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regional astrocyte dysregulation and altered glymphatic\u2010related markers in Alzheimer's disease frontal cortex"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8672-7619","authenticated-orcid":false,"given":"Harry","family":"Alexopoulos","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Biophysics Faculty of Biology National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xanthippi P.","family":"Louka","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Biophysics Faculty of Biology National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo Rosario","family":"De Natale","sequence":"additional","affiliation":[{"name":"Neurodegeneration Imaging Group University of Exeter Medical School  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Koutroubi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Biophysics Faculty of Biology National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Cashmore","sequence":"additional","affiliation":[{"name":"Neurodegeneration Imaging Group University of Exeter Medical School  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria 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