{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T15:57:51Z","timestamp":1785859071753,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"am","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2015M580755"],"award-info":[{"award-number":["2015M580755"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Backgrounds<\/jats:title><jats:p>Streptozotocin (STZ)\u2010 induced diabetic monkey is a wide used preclinical animal model for the investigation of diabetes such as islet transplantation and development of diabetic drugs. There are serious side effects of this method, including nausea, emesis, weight loss, liver damage, renal failure, and metabolic acidosis. In order to reduce the side effects, diabetic monkeys were induced using clinical\u2010grade STZ. However, clinical\u2010grade STZ is not available in China. Here, we establised a method by using 100 mg\/kg analytical\u2010grade STZ to induce complete diabetes in cynomolgus monkey without generating adverse effects to liver and renal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three cynomolgus monkeys were used in this study. 100\u00a0mg\/kg STZ dissolved in normal saline and infused through vein line in 5\u00a0minutes after indwelling catheter in the carotid artery and jugular vein. After the STZ administration, blood glucose levels were examined every 1 or 2\u00a0hours in the first 48\u00a0hours. Then, blood glucose levels were examined twice per day during the first week after the STZ injection. Insulin and C\u2010peptide levels were measured by ELISA. Blood chemistry of hepatic and renal function tests were performed. Insulin and glucagon expression in the islet of diabetic monkey and normal monkey were examined by immunohistochemistry assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The stimulated C\u2010peptide level (Intravenous glucose tolerance test) which is less than 0.5 ng\/mL, the triphasic blood glucose response and the destroyed \u03b2 cell suggested the complete induction of diabetes model. No apparent adverse effects were observed including no signs of vomiting and toxicity after STZ injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In summary, we established a safe and reproducible STZ\u2010induced diabetic cynomolgus monkey model for islet transplantation which will be used to develop novel approaches for the treatment of diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12109","type":"journal-article","created":{"date-parts":[[2020,3,21]],"date-time":"2020-03-21T05:14:52Z","timestamp":1584767692000},"page":"79-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Induction of diabetes in cynomolgus monkey with one shot of analytical grade streptozotocin"],"prefix":"10.1002","volume":"3","author":[{"given":"Zhengzhao","family":"Liu","sequence":"first","affiliation":[{"name":"Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational Medicine Shenzhen Second People's Hospital The First Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"},{"name":"Movement System Injury and Repair Research Center Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Lu","sequence":"additional","affiliation":[{"name":"Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational Medicine Shenzhen Second People's Hospital The First Affiliated Hospital of Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbao","family":"Hu","sequence":"additional","affiliation":[{"name":"Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational Medicine Shenzhen Second People's Hospital The First Affiliated Hospital of Shenzhen University Health 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