{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:51:20Z","timestamp":1780469480563,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AR070851\u201001A1"],"award-info":[{"award-number":["R01 AR070851\u201001A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Wound healing can result in various outcomes, including hypertrophic scar (HTS). Pigs serve as models to study wound healing as their skin shares physiologic similarity with humans. Yorkshire (Yk) and Duroc (Dc) pigs have been used to mimic normal and abnormal wound healing, respectively. The reason behind this differential healing phenotype was explored here.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98\u00a0days. PCR arrays were used to determine differential gene expression. Vancouver Scar Scale (VSS) scores were given. Re\u2010epithelialization was analyzed. H&amp;E, Mason's trichrome, and immunostains were used to determine cellularity, collagen content, and blood vessel density, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Yk wounds heal to a \u201cport wine\u201d HTS, resembling scarring in Fitzpatrick skin types (FST) I\u2010III. Dc wounds heal to a dyspigmented, non\u2010pliable HTS, resembling scarring in FST IV\u2013VI. Gene expression during wound healing was differentially regulated versus uninjured skin in 40\/80 genes, 15 of which differed between breeds. Yk scars had a higher VSS score at all time points. Yk and Dc wounds had equivalent re\u2010epithelialization, collagen disorganization, and blood vessel density.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings demonstrate that Dc and Yk pigs can produce HTS. Wound creation and healing were consistent among breeds, and differences in gene expression were not sufficient to explain differences in resulting scar phenotype. Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12188","type":"journal-article","created":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T06:38:31Z","timestamp":1637649511000},"page":"406-417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In\u2010depth examination of hyperproliferative healing in two breeds of <i>Sus scrofa domesticus<\/i> commonly used for research"],"prefix":"10.1002","volume":"4","author":[{"given":"Colton H.","family":"Funkhouser","sequence":"first","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey W.","family":"Shupp","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington District of Columbia USA"},{"name":"Department of Biochemistry and Molecular and Cellular Biology Georgetown University Medical Center Washington District of Columbia USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington District of Columbia USA"},{"name":"The Burn Center Department of Surgery MedStar Washington Hospital Center Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2292-4960","authenticated-orcid":false,"given":"Bonnie C.","family":"Carney","sequence":"additional","affiliation":[{"name":"Firefighters' Burn and Surgical Research Laboratory MedStar Health Research Institute Washington District of Columbia USA"},{"name":"Department of Biochemistry and Molecular and Cellular Biology Georgetown University Medical Center Washington District of Columbia USA"},{"name":"Department of Surgery Georgetown University School of Medicine Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030711"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013e3182374452"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsc.2014.04.010"},{"key":"e_1_2_8_5_1","first-page":"1004","article-title":"Incidence of hypertrophic scars among African Americans linked to vitamin D\u20103 metabolism?","volume":"97","author":"Cooke GL","year":"2005","journal-title":"J Natl Med 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