{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:47:08Z","timestamp":1793692028470,"version":"4.5.1"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T00:00:00Z","timestamp":1646956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The objective of this study was to validate an animal model for dry eye during and after the administration of 1% ophthalmic atropine sulfate (OAS) in New Zealand white (NZW) rabbits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>OAS (1%) was applied three times per day to 30 eyes of 15\u00a0healthy NZW rabbits. Sacrifice, enucleation, and lacrimal gland removal took place on days 15, 21, and 30 (OAS group). A second group (n\u00a0=\u00a05) was used as control. Clinical evaluations took place on days 3, 10, 15, 18, 21, 24 and 30. The primary endpoints were: Schirmer I test, tear break\u2010up time (TBUT), and corneal fluorescein staining. As secondary endpoints, clinical changes including intraocular pressure, and histopathology were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      While OAS was administered, the Schirmer I test showed a statistically significant reduction for OAS group versus control (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), and versus basal production (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). TBUT showed statistically significant differences between groups (days 3 and 10;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.001) and versus basal values (day 3;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). Fluorescein staining showed a statistically significant difference (day 3;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.001). The most frequent clinical finding was conjunctival hyperemia (76.9% OAS vs. 20% control). For histopathology, all OAS subjects presented some degree of inflammation (86.7% minimal; 13.3% mild) whereas the control presented only 30% minimal inflammation. Goblet cell density showed no difference.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The effectiveness of the OAS dry eye model in NZW rabbits as reported in previous studies was confirmed, provided that the application of the drug is maintained throughout the intervention; it is not a viable model after OAS administration is suspended.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.12218","type":"journal-article","created":{"date-parts":[[2022,3,12]],"date-time":"2022-03-12T00:04:47Z","timestamp":1647043487000},"page":"266-273","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Validation of a preclinical dry eye model in New Zealand white rabbits during and following topical instillation of 1% ophthalmic atropine sulfate"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8034-8071","authenticated-orcid":false,"given":"Alejandra","family":"S\u00e1nchez\u2010R\u00edos","sequence":"first","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elba Yadira","family":"Correa\u2010Gallegos","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Medina\u2010Espinoza","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Anaid","family":"Navarro\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Olvera\u2010Monta\u00f1o","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo","family":"Baiza\u2010Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-1218","authenticated-orcid":false,"given":"Patricia","family":"Mu\u00f1oz\u2010Villegas","sequence":"additional","affiliation":[{"name":"Medical Affairs Department Laboratorios Sophia, SA de CV Zapopan Jalisco Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1407936"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095\u2010019\u20102588\u20105"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03\u20101055"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4368"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00280"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01240"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000055537"},{"issue":"9","key":"e_1_2_9_10_1","first-page":"305","article-title":"Effects of ALY688 on atropine\u2010induced dry eye in rabbits","volume":"60","author":"Crawford 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Accessed January 3 2022."}],"container-title":["Animal Models and Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.12218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ame2.12218","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.12218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T00:15:21Z","timestamp":1726791321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ame2.12218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,11]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1002\/ame2.12218"],"URL":"https:\/\/doi.org\/10.1002\/ame2.12218","archive":["Portico"],"relation":{},"ISSN":["2576-2095","2576-2095"],"issn-type":[{"value":"2576-2095","type":"print"},{"value":"2576-2095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,11]]},"assertion":[{"value":"2021-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}