{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:26:16Z","timestamp":1790508376628,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We aimed to reveal the mechanism of functional constipation in the treatment of <jats:italic>Atractylodes macrocephala<\/jats:italic> Koidz. (AMK) and <jats:italic>Paeonia lactiflora<\/jats:italic> Pall. (PLP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The main active ingredients of AMK and PLP were screened by the Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform. A database of functional constipation targets was established by GeneCard and OMIM. An \u201cingredient\u2010target\u201d network map was constructed with Cytoscape software (version 3.7.1), and molecular docking analysis was performed on the components and genes with the highest scores. The rats in the normal group were given saline, and those in the other groups were given 10\u00a0mg\/kg diphenoxylate once a day for 14\u2009days. The serum and intestinal tissue levels of adenosine monophosphate (cAMP), protein kinase A (PKA), and adenylyl cyclase (AC) of the rats and aquaporin (AQP)1, AQP3, and AQP8 were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AMK and PLP had a significant role in the regulation of targets in the treatment of functional constipation. After treatment with AMK, PLP, or mosapride, the serum and intestinal tissue levels of AC, cAMP, and PKA were significantly downregulated. Groups receiving AMK and PLP or mosapride exhibited a reduction in the level of AQP1, AQP3, and AQP8 to varying degrees.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Molecular docking analysis revealed that AMK and PLP had a significant role in the regulation of targets in the treatment of functional constipation. Studies have confirmed that AMK and PLP can also affect AC, cAMP, and PKA. AC, cAMP, and PKA in model rats were significantly downregulated. AQP expression is closely related to AC, cAMP, and PKA. AMK and PLP can reduce the expression of AQP1, AQP3, and AQP9 in the colon of constipated rats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12226","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T09:20:23Z","timestamp":1650619223000},"page":"120-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Network pharmacological prediction and molecular docking analysis of the combination of <i>Atractylodes macrocephala<\/i> Koidz. and <i>Paeonia lactiflora<\/i> Pall. in the treatment of functional constipation and its verification"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6834-1889","authenticated-orcid":false,"given":"Yuxiao","family":"Meng","sequence":"first","affiliation":[{"name":"School of Pharmacy Zhejiang Chinese Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zhejiang Chinese Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zhejiang Chinese Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zhejiang Chinese Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Zhejiang Chinese Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0222-y"},{"key":"e_1_2_9_3_1","first-page":"919","article-title":"Epidemiology and management of chronic constipation in elderly patients","volume":"10","author":"Vazquez Roque M","year":"2015","journal-title":"Clin Interv Aging"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"4","article-title":"Differential diagnosis of abdominal masses in children","volume":"51","author":"Sung IK","year":"2008","journal-title":"Korean J Gastroenterol"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-019-03283-5"},{"issue":"6","key":"e_1_2_9_6_1","first-page":"757","article-title":"Treatment of irritable bowel syndrome by Chinese medicine and pharmacy: an analysis of data mining on experiences of experts","volume":"33","author":"Zhang BH","year":"2013","journal-title":"Zhongguo Zhong Xi Yi Jie He Za Zhi"},{"key":"e_1_2_9_7_1","first-page":"158","volume-title":"The Pharmacopoeia of the People\u2019s Republic of China [S]. 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