{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T09:02:24Z","timestamp":1768294944941,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870042"],"award-info":[{"award-number":["81870042"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["21ZR1453800"],"award-info":[{"award-number":["21ZR1453800"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Aberrant expression of microRNAs (miRNAs) has been associated with the pathogenesis of pulmonary hypertension (PH). It is, however, not clear whether miRNAs are involved in estrogen rescue of PH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fresh plasma samples were prepared from 12 idiopathic pulmonary arterial hypertension (IPAH) patients and 12 healthy controls undergoing right heart catheterization in Shanghai Pulmonary Hospital. From each sample, 5\u2009\u03bcg of total RNA was tagged and hybridized on microRNA microarray chips. Monocrotaline\u2010induced PH (MCT\u2010PH) male rats were treated with 17\u03b2\u2010estradiol (E<jats:sub>2<\/jats:sub>) or vehicle. Subgroups were cotreated with estrogen receptor (ER) antagonist or with antagonist of miRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Many circulating miRNAs, including miR\u201021\u20105p and miR\u2010574\u20105p, were markedly expressed in patients and of interest in predicting mean pulmonary arterial pressure elevation in patients. The expression of miR\u201021\u20105p in the lungs was significantly upregulated in MCT\u2010PH rats compared with the controls. However, miR\u2010574\u20105p showed no difference in the lungs of MCT\u2010PH rats and controls. miR\u201021\u20105p was selected for further analysis in rats as E<jats:sub>2<\/jats:sub> strongly regulated it. E<jats:sub>2<\/jats:sub> decreased miR\u201021\u20105p expression in the lungs of MCT\u2010PH rats by ER\u03b2. E<jats:sub>2<\/jats:sub> reversed miR\u201021\u20105p target gene FilGAP downregulation in the lungs of MCT\u2010PH rats. The abnormal expression of RhoA, ROCK2, Rac1 and c\u2010Jun in the lungs of MCT\u2010PH rats was inhibited by E<jats:sub>2<\/jats:sub> and miR\u201021\u20105p antagonist.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miR\u201021\u20105p level was remarkably associated with PH severity in patients. Moreover, the miR\u201021\u20105p\/FilGAP signaling pathway modulated the protective effect of E<jats:sub>2<\/jats:sub> on MCT\u2010PH through ER\u03b2.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12253","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T10:14:02Z","timestamp":1655460842000},"page":"217-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Role of <scp>miR<\/scp>\u201021\u20105p\/<scp>FilGAP<\/scp> axis in estradiol alleviating the progression of monocrotaline\u2010induced pulmonary hypertension"],"prefix":"10.1002","volume":"5","author":[{"given":"Xiaoyi","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Cardio\u2010Pulmonary Circulation Shanghai Pulmonary Hospital School of Medicine Tongji University  Shanghai 200433 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