{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T04:35:01Z","timestamp":1776141301151,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Chukrasia velutina<\/jats:italic> is an enthnomedicinally used plant reported to have significant medicinal values. The present study aimed to explore the pharmacological activities of bark methanol extract using in vitro, in vivo and in silico models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was designed to investigate the pharmacological effects of methanol extract of <jats:italic>Chukrasia velutina<\/jats:italic> bark (MECVB) through in vitro, in vivo and in silico assays. Analgesic activity was tested using formalin\u2010induced nociception and acetic acid\u2010induced writhing assays while the antipyretic effect was tested using yeast\u2010induced hyperthermia in mice model. The antioxidant effect was tested using the DPPH and reducing power assay and the cytotoxic screening was tested using the brine shrimp lethality bioassay. In addition, in silico studies were conducted using computer aided methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the acetic acid\u2010induced writhing assay, the extract showed 28.36% and 56.16% inhibition of writhing for doses of 200 and 400\u2009mg\/kg, respectively. Moreover, a dose\u2010dependent formalin\u2010induced licking response was observed in both early and late phase. In yeast\u2010induced pyrexia, the MECVB exhibited (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) antipyretic effect. The extract demonstrated an IC<jats:sub>50<\/jats:sub> value of 78.86\u2009\u03bcg\/ml compared with ascorbic acid (IC<jats:sub>50<\/jats:sub> 23.53\u2009\u03bcg\/ml) in the DPPH scavenging assay. The compounds sitosterol, 5,7\u2010dimethoxycoumarin and scopoletin were seen be effective in molecular docking scores against COX\u2010I (2OYE), COX\u2010II (6COX) and human peroxiredoxin 5 (1HD2). In ADME\/T analysis, 5,7\u2010dimethoxycoumarin and scopoletin satisfied Lipinski's rule of five and thus are potential drug candidates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The bark of <jats:italic>Chukrasia velutina<\/jats:italic> showed significant analgesic and antipyretic properties and is a potential source of natural anti\u2010oxidative agents.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12268","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T09:27:54Z","timestamp":1662024474000},"page":"377-388","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pharmacological insights into <i>Chukrasia velutina<\/i> bark: Experimental and computer\u2010aided approaches"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8834-1755","authenticated-orcid":false,"given":"Israt","family":"Jahan","sequence":"first","affiliation":[{"name":"Department of Pharmacy International Islamic University Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahenur Alam","family":"Sakib","sequence":"additional","affiliation":[{"name":"Department of Pharmacy International Islamic University Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najmul","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy International Islamic University Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohuya","family":"Majumder","sequence":"additional","affiliation":[{"name":"Department of Pharmacy East West University  Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjida","family":"Sharmin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy International Islamic University Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1457-0245","authenticated-orcid":false,"given":"A. 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