{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:21:48Z","timestamp":1747146108383,"version":"3.40.5"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The dorsomedial periaqueductal gray (dmPAG) is a mesencephalic area and has numerous functions including cardiovascular regulation. Because nitric oxide (NO) is present in the dmPAG, here we investigate, the probable cardiovascular effect of NO in the dmPAG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Five groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a06 for each group) were used as follows: (1) control; (2) L\u2010NAME (<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010nitro\u2010L\u2010arginine methyl ester, a NO synthase inhibitor, 90\u2009nmol); (3) L\u2010arginine (L\u2010Arg, a precursor for NO, 60\u2009nmol); (4) Sodium nitroprusside (SNP, a NO donor, 27\u2009nmol); and (5) L\u2010Arg\u2009+\u2009L\u2010NAME. The cardiovascular parameters were recorded by a Power Lab device after cannulation of the femoral artery. Drugs were injected using a stereotaxic instrument. The changes (\u2206) in systolic blood pressure (SBP), mean arterial pressure (MAP), and heart rate (HR) were calculated at different times and compared to the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Microinjection of L\u2010NAME significantly increased \u2206SBP, \u2206MAP, and \u2206HR more than saline (from <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 to <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). L\u2010Arg only significantly increased \u2206HR (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In the L\u2010Arg\u2009+\u2009L\u2010NAME group, the above parameters also significantly increased (from <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01 to <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) but not as significantly as with L\u2010NAME alone. Microinjection of SNP significantly decreased \u2206SBP and \u2206MAP more than in the control and L\u2010NAME groups (from <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01 to <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), but \u2206HR did not change significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results indicated that NO in dmPAG has an inhibitory effect on cardiovascular responses in anesthetized rats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12292","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T05:51:17Z","timestamp":1669182677000},"page":"557-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The role of nitric oxide in the dorsomedial periaqueductal gray (<scp>dmPAG<\/scp>) column in cardiovascular responses in urethane\u2010anesthetized male rats"],"prefix":"10.1002","volume":"5","author":[{"given":"Mohammad","family":"Najaftomaraei","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Mashhad University of Medical Sciences  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atiyeh","family":"Ghorbani","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Mashhad University of Medical Sciences  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Material Science and Metallurgy Engineering Islamic Azad University \u2010 Karaj Branch  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1645-7094","authenticated-orcid":false,"given":"Reza","family":"Mohebbati","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Gonabad University 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