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Aurora B, a key mitotic regulator, has been reported to be involved in the progression of various tumors, yet its role in CCA is still unclarified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human CCA tissues and murine spontaneous CCA models were used to assess Aurora B expression in CCA. A loss\u2010of\u2010function model was constructed in CCA cells to determine the role of Aurora B in CCA progression. Subcutaneous and liver orthotopic xenograft models were used to assess the therapeutic potential of Aurora B inhibitors in CCA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In murine spontaneous CCA models, Aurora B was significantly upregulated. Elevated Aurora B expression was also observed in 62.3% of human specimens in our validation cohort (143 CCA specimens), and high Aurora B expression was positively correlated with pathological parameters of tumors and poor survival. Knockdown of Aurora B by siRNA and heteroduplex oligonucleotide (HDO) or an Aurora B kinase inhibitor (AZD1152) significantly suppressed CCA progression via G2\/M arrest induction. An interaction between Aurora B and c\u2010Myc was found in CCA cells. Targeting Aurora B significantly reduced this interaction and accelerated the proteasomal degradation of c\u2010Myc, suggesting that Aurora B promoted the malignant properties of CCA by stabilizing c\u2010Myc. Furthermore, sequential application of AZD1152 or Aurora B HDO drastically improved the efficacy of gemcitabine in CCA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Aurora B plays an essential role in CCA progression by modulating c\u2010Myc stability and represents a new target for treatment and chemosensitization in CCA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12370","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T08:05:35Z","timestamp":1705910735000},"page":"626-640","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aurora B facilitates cholangiocarcinoma progression by stabilizing <scp>c\u2010Myc<\/scp>"],"prefix":"10.1002","volume":"7","author":[{"given":"Ke","family":"Liu","sequence":"first","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou 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