{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:22:10Z","timestamp":1747146130534,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31301137","82174020"],"award-info":[{"award-number":["31301137","82174020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of Claudin\u20101 in tongue squamous cell carcinoma (TSCC) metastasis needs further clarification, particularly its impact on cell migration. Herein, our study aims to investigate the role of Claudin\u20101 in TSCC cell migration and its underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>36 TSCC tissue samples underwent immunohistochemical staining for Claudin\u20101. Western blotting and immunofluorescence analyses were conducted to evaluate Claudin\u20101 expression and distribution in TSCC cells. Claudin\u20101 knockdown cell lines were established using short hairpin RNA transfection. Migration effects were assessed through wound healing assays. Furthermore, the expression of EMT\u2010associated molecules was measured via western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Claudin\u20101 expression decreased as TSCC malignancy increased. Adenosine monophosphate\u2013activated protein kinase (AMPK) activation led to increased Claudin\u20101 expression and membrane translocation, inhibiting TSCC cell migration and epithelial\u2013mesenchymal transition (EMT). Conversely, Claudin\u20101 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results indicated that AMPK activation suppresses TSCC cell migration by targeting Claudin\u20101 and EMT pathways.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12444","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T13:41:53Z","timestamp":1721223713000},"page":"606-616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The activation of adenosine monophosphate\u2013activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin\u20101 via epithelial\u2013mesenchymal 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