{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T02:52:11Z","timestamp":1768359131873,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81160012"],"award-info":[{"award-number":["81160012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560301"],"award-info":[{"award-number":["81560301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Under hypoxia, exaggerated compensatory responses may lead to acute mountain sickness. The excessive vasodilatory effect of nitric oxide (NO) can lower the hypoxic pulmonary vasoconstriction (HPV) and peripheral blood pressure. While NO is catalyzed by various nitric oxide synthase (NOS) isoforms, the regulatory roles of these types in the hemodynamics of pulmonary and systemic circulation in living hypoxic animals remain unclear. Therefore, this study aims to investigate the regulatory effects of different NOS isoforms on pulmonary and systemic circulation in hypoxic rats by employing selective NOS inhibitors and continuously monitoring hemodynamic parameters of both pulmonary and systemic circulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty healthy male Sprague\u2013Dawley (SD) rats were randomly divided into four groups: Control group (<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010nitro\u2010D\u2010arginine methyl ester, D\u2010NAME), L\u2010NAME group (non\u2010selective NOS inhibitor, <jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010nitro\u2010L\u2010arginine methyl ester), AG group (inducible NOS inhibitor group, aminoguanidine), and 7\u2010NI group (neurological NOS inhibitor, 7\u2010nitroindazole). Hemodynamic parameters of rats were monitored for 10\u2009min after inhibitor administration and 5\u2009min after induction of hypoxia [15% O<jats:sub>2<\/jats:sub>, 2200\u2009m a. sl., 582\u2009mmHg (76.5\u2009kPa), Xining, China] using the real\u2010time dynamic monitoring model for pulmonary and systemic circulation hemodynamics in\u00a0vivo. Serum NO concentrations and blood gas analysis were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under normoxia, mean arterial pressure and total peripheral vascular resistance were increased, and ascending aortic blood flow and serum NO concentration were decreased in the L\u2010NAME and AG groups. During hypoxia, pulmonary arterial pressure and pulmonary vascular resistance were significantly increased in the L\u2010NAME and AG groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This compensatory mechanism activated by inducible NOS and endothelial NOS effectively counteracts the pulmonary hemodynamic changes induced by hypoxic stress. It plays a crucial role in alleviating hypoxia\u2010induced pulmonary arterial hypertension.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12453","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T09:16:41Z","timestamp":1718702201000},"page":"344-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of different nitric oxide synthases on pulmonary and systemic hemodynamics in hypoxic stress rat model"],"prefix":"10.1002","volume":"8","author":[{"given":"Huan","family":"Zhang","sequence":"first","affiliation":[{"name":"Research Center for High Altitude Medicine Qinghai University  Xining Qinghai China"},{"name":"Department of Pathology The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine 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